Content Determination of Phenylpropanoids and Enhancing Exercise Ability of Effective Fractions in Pedicularis densispica.

BACKGROUND

Most researches were focused on chemical constituents and bioactivities of . However, there were a few reports on simultaneous determination of the series phenylpropanoids compounds in by High Performance Liquid Chromatography (HPLC).

OBJECTIVE

To establish an HPLC method for simultaneous determination of salidroside, verbascoside, iso-verbascoside, leucoseptoside A, jionoside D and martynoside in (PD), and to assess the enhancing exercise ability of effective fractions of phenylpropanoids (EFP).

MATERIALS AND METHODS

The separation was performed on C column with step-wise gradient elution with water (A)-methanol (B) as the mobile phase at a flow rate of 1.0 mL/min, with detection wavelength at 275 nm (0-4 min) and 330 nm (4-40 min). The EFP were obtained from extracts of PD by resin gradient dilution. The enhancing exercise ability of EFP was exerted in exhaustive swimming and anoxia endurance tests .

RESULTS

The contents of six marker compounds had good linear relationship in the ranges of 2.10-8.40, 13.60-54.40, 0.93-3.72, 0.53-2.12, 1.50-6.00, 0.37-1.28, respectively, and the average recoveries of the six phenylpropanoids were all in the range of 98-103%. Total contents of phenylpropanoids in EFP were more than 60%. Three medicine groups of exhaustive swimming and anoxia endurance time were higher than those of the water group.

CONCLUSION

The analytical method is reliable, simple and accurate, and can be used for the comprehensive quality control of PD. This experiment suggests that PD has the effect of promoting the recovery and elimination of fatigue and improving the exercise capacity.

SUMMARY

A simple, practical and low-cost RP-HPLC method has been developed for the simultaneous determination of six marker phenylpropanoids .Three effective fractions of phenylpropanoids groups of exhaustive swimming and anoxia endurance time were higher than those of the water group.The separation was performed on C18 column with stepwise gradient elution with water-methanol. The enhancing exercise ability was exerted in exhaustive swimming and anoxia endurance tests .This plant has the effect of promoting the recovery and elimination of fatigue and improving the exercise capacity. PD: Pedicularis densispica, EFP: Effective fractions of phenylpropanoids, DAD: Diode array detector, HPLC: High performance liquid chromatography, LOD: Limits of detection, LOQ: Limits of quantification, RSD: Relative standard deviation, BV: Bed volumes.