Chu Hongbiao, Zhang Zhihua, Chen Dong, Wang Xi, Tu Qilong
Department of Pharmacy, School of Medicine, Jinggangshan University, Jiangxi, P.R. China.
Pharmacogn Mag. 2017 Apr-Jun;13(50):230-235. doi: 10.4103/0973-1296.204567. Epub 2017 Apr 18.
Most researches were focused on chemical constituents and bioactivities of . However, there were a few reports on simultaneous determination of the series phenylpropanoids compounds in by High Performance Liquid Chromatography (HPLC).
To establish an HPLC method for simultaneous determination of salidroside, verbascoside, iso-verbascoside, leucoseptoside A, jionoside D and martynoside in (PD), and to assess the enhancing exercise ability of effective fractions of phenylpropanoids (EFP).
The separation was performed on C column with step-wise gradient elution with water (A)-methanol (B) as the mobile phase at a flow rate of 1.0 mL/min, with detection wavelength at 275 nm (0-4 min) and 330 nm (4-40 min). The EFP were obtained from extracts of PD by resin gradient dilution. The enhancing exercise ability of EFP was exerted in exhaustive swimming and anoxia endurance tests .
The contents of six marker compounds had good linear relationship in the ranges of 2.10-8.40, 13.60-54.40, 0.93-3.72, 0.53-2.12, 1.50-6.00, 0.37-1.28, respectively, and the average recoveries of the six phenylpropanoids were all in the range of 98-103%. Total contents of phenylpropanoids in EFP were more than 60%. Three medicine groups of exhaustive swimming and anoxia endurance time were higher than those of the water group.
The analytical method is reliable, simple and accurate, and can be used for the comprehensive quality control of PD. This experiment suggests that PD has the effect of promoting the recovery and elimination of fatigue and improving the exercise capacity.
A simple, practical and low-cost RP-HPLC method has been developed for the simultaneous determination of six marker phenylpropanoids .Three effective fractions of phenylpropanoids groups of exhaustive swimming and anoxia endurance time were higher than those of the water group.The separation was performed on C18 column with stepwise gradient elution with water-methanol. The enhancing exercise ability was exerted in exhaustive swimming and anoxia endurance tests .This plant has the effect of promoting the recovery and elimination of fatigue and improving the exercise capacity. PD: Pedicularis densispica, EFP: Effective fractions of phenylpropanoids, DAD: Diode array detector, HPLC: High performance liquid chromatography, LOD: Limits of detection, LOQ: Limits of quantification, RSD: Relative standard deviation, BV: Bed volumes.
大多数研究集中于[具体研究对象]的化学成分和生物活性。然而，关于采用高效液相色谱法（HPLC）同时测定[具体研究对象]中一系列苯丙素类化合物的报道较少。
建立一种HPLC法同时测定密花马先蒿（Pedicularis densispica，PD）中红景天苷、毛蕊花糖苷、异毛蕊花糖苷、柳穿鱼叶苷A、红轮千里光苷D和肉叶云香苷的含量，并评价苯丙素类有效部位（EFP）增强运动能力的作用。
采用C18柱，以水（A）-甲醇（B）为流动相进行梯度洗脱，流速为1.0 mL/min，检测波长为275 nm（0～4分钟）和330 nm（4～40分钟）。通过树脂梯度稀释从PD提取物中获得EFP。在力竭游泳和耐缺氧耐力试验中发挥EFP增强运动能力的作用。
6种指标性成分在2.10～8.40、13.60～54.40、0.93～3.72、0.53～2.12、1.50～6.00、0.37～1.28范围内线性关系良好，6种苯丙素类成分的平均回收率均在98%～103%之间。EFP中苯丙素类成分的总含量超过60%。力竭游泳和耐缺氧3个给药组的时间均高于水对照组。
该分析方法可靠、简便、准确，可用于PD的综合质量控制。本实验提示PD具有促进疲劳恢复和消除、提高运动能力的作用。
建立了一种简便、实用、低成本的RP-HPLC法同时测定6种指标性苯丙素类成分。苯丙素类3个有效部位组的力竭游泳和耐缺氧时间均高于水对照组。采用C18柱，以水-甲醇进行梯度洗脱。在力竭游泳和耐缺氧耐力试验中发挥增强运动能力的作用。该植物具有促进疲劳恢复和消除、提高运动能力的作用。PD：密花马先蒿，EFP：苯丙素类有效部位，DAD：二极管阵列检测器，HPLC：高效液相色谱法；LOD：检测限，LOQ：定量限，RSD：相对标准偏差，BV：柱体积
