Ma Chaoyang, Hu Liming, Tao Guanjun, Lv Wenping, Wang Hongxin
State Key Laboratory of Food Science and Technology (Jiangnan University), School of Food Science and Technology, 1800 Lihu Road, 214122 Wuxi, China.
Air Force Logistics College, Xuzhou, Jiangshu 221006, China.
J Pharm Biomed Anal. 2015 Feb;105:84-90. doi: 10.1016/j.jpba.2014.11.036. Epub 2014 Dec 4.
An ultra-performance liquid chromatography-quadrupole time-of-flight-based metabolomic approach was developed to study influence of salidroside, an anti-fatigue ingredient from Rhoiola rosea, on urinary metabolic profiling of rats to a single dose of 180 mg/kg per day. Unsupervised principal component analysis (PCA) and supervised orthogonal pre-projection to latent structures discriminate analysis (OPLS-DA) on metabolite profiling revealed obvious differentiation between the salidroside treated groups and controls in both positive and negative ion modes. Eleven urinary metabolites contributing to the differentiation were identified as anti-fatigue biomarkers: N-acetylserotonin, 2-Methoxyestrone 3-glucuronide, Taurine, Melatonin, Sorbitol, Geranyl diphosphate, Z-nucleotide, Cortisone, Dihydrocortisol, Sebacic acid, Pregnenolone sulfate. The physiological significance of these biomarkers is discussed. The work showed that metabolomics is a powerful tool in studying the anti-fatigue effects of natural compound salidroside on multiple targets in vivo.
开发了一种基于超高效液相色谱-四极杆飞行时间的代谢组学方法，以研究红景天中的抗疲劳成分红景天苷对大鼠尿液代谢谱的影响，每日单剂量为180 mg/kg。对代谢物谱进行无监督主成分分析（PCA）和有监督的正交投影到潜在结构判别分析（OPLS-DA），结果显示在正离子和负离子模式下，红景天苷处理组与对照组之间均有明显差异。确定了导致这种差异的11种尿液代谢物为抗疲劳生物标志物：N-乙酰血清素、2-甲氧基雌酮3-葡萄糖醛酸苷、牛磺酸、褪黑素、山梨醇、香叶基二磷酸、Z-核苷酸、可的松、双氢皮质醇、癸二酸、孕烯醇酮硫酸盐。讨论了这些生物标志物的生理意义。这项工作表明，代谢组学是研究天然化合物红景天苷在体内多靶点抗疲劳作用的有力工具。
