来自……的三个类甜蛋白基因的克隆与表达
Cloning and expression of three thaumatin-like protein genes from .
作者信息
Liu Mengmeng, Zhang Dawei, Xing Yongmei, Guo Shunxing
机构信息
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, China.
出版信息
Acta Pharm Sin B. 2017 May;7(3):373-380. doi: 10.1016/j.apsb.2017.03.001. Epub 2017 Apr 11.
Genes encoding thaumatin-like protein () are frequently found in fungal genomes. However, information on genes in is still limited. In this study, three genes were cloned from . The full-length coding sequence of , and were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that , and were clustered with sequences from and , respectively. The expression patterns of the three genes were higher in with infection than in the sclerotia without . Furthermore, over-expression of three PuTLPs were carried out in BL21 (DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that , and in may be involved in the defense response to infections.
编码类甜蛋白（thaumatin-like protein，TLP）的基因在真菌基因组中经常被发现。然而，关于稻瘟病菌（Magnaporthe oryzae）中TLP基因的信息仍然有限。在本研究中，从稻瘟病菌中克隆了三个TLP基因。MoTLP1、MoTLP2和MoTLP3的全长编码序列分别为768、759和561 bp，分别编码256、253和187个氨基酸。系统发育树表明，MoTLP1、MoTLP2和MoTLP3分别与来自灰葡萄孢（Botrytis cinerea）和核盘菌（Sclerotinia sclerotiorum）的序列聚类。这三个TLP基因在稻瘟病菌感染的菌株中的表达模式高于未感染的菌核。此外，在大肠杆菌BL21 (DE3) 菌株中对三个稻瘟病菌TLP进行了过表达，并使用Ni-NTA树脂获得了可用于制备特异性抗体的高质量蛋白质。这些结果表明，稻瘟病菌中的MoTLP1、MoTLP2和MoTLP3可能参与了对稻瘟病菌感染的防御反应。
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