Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100193, China.
Sci Rep. 2017 Jun 26;7(1):4226. doi: 10.1038/s41598-017-04578-9.
It has been well established that some Armillaria species are symbionts of Polyporus umbellatus, However, little is known about the evolutionary history of P. umbellatus-Armillaria associations. In this research, we used an analysis based on the strength of the phylogenetic signal to investigate P. umbellatus-Armillaria associations in 57 sclerotial samples across 11 provinces of China. We isolated Armillaria strains from the invasion cavity inside the sclerotia of P. umbellatus and then phylogenetically analyzed these Armillaria isolates. We also tested the effect of P. umbellatus and Armillaria phylogenies on the P. umbellatus-Armillaria associations. We isolated forty-seven Armillaria strains from 26 P. umbellatus sclerotial samples. All Armillaria isolates were classified into the 5 phylogenetic lineages found in China except for one singleton. Among the 5 phylogenetic lineages, one lineage (lineage 8) was recognized by delimitation of an uncertain phylogenetic lineage in previous study. Results of simple Mantel test implied that phylogenetically related P. umbellatus populations tend to interact with phylogenetically related Armillaria species. Phylogenetic network analyses revealed that the interaction between P. umbellatus and Armillaria is significantly influenced by the phylogenetic relationships between the Armillaria species.
已经证实，一些蜜环菌物种是香菇的共生菌。然而，关于香菇与蜜环菌的进化历史知之甚少。在这项研究中，我们使用基于系统发育信号强度的分析方法，研究了中国 11 个省份的 57 个菌核样本中的香菇-蜜环菌共生关系。我们从香菇菌核内部的入侵腔中分离出蜜环菌菌株，然后对这些蜜环菌分离株进行系统发育分析。我们还测试了香菇和蜜环菌系统发育对香菇-蜜环菌共生关系的影响。我们从 26 个香菇菌核样本中分离出 47 株蜜环菌菌株。除了一个单倍型外，所有的蜜环菌分离株都被归入了中国发现的 5 个系统发育谱系中。在这 5 个系统发育谱系中，有一个谱系（谱系 8）是在前一次研究中通过不确定的系统发育谱系的划分来识别的。简单 Mantel 检验的结果表明，系统发育上相关的香菇种群往往与系统发育上相关的蜜环菌物种相互作用。系统发育网络分析表明，香菇与蜜环菌的相互作用受到蜜环菌物种之间的系统发育关系的显著影响。
