Amiri Ali, Sabooteh Toomaj, Shahsavar Farhad, Anbari Khatereh, Pouremadi Flora
Department of Internal Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Genom Data. 2017 May 4;12:146-150. doi: 10.1016/j.gdata.2017.05.005. eCollection 2017 Jun.
Tuberculosis (TB) is caused by infection of Mycobacterium tuberculosis. Host genetic variability is an important determinant of the risk of developing TB in humans. Although the association between polymorphisms and TB has been studied in various populations, the results are controversial. The aim of this study was to investigate mannose-binding lectin () gene polymorphisms with susceptibility to pulmonary tuberculosis (PTB) in a Lur population of Iran.
In this case-control study, four functional gene polymorphisms (, , and ) were genotyped by using PCR Single Strand Conformation Polymorphism (SSCP) technique in a Lur population living in Lorestan Province, consisting of 100 patients with pulmonary tuberculosis (PTB) age and sex matched 100 healthy controls (HCs). Association analyses were performed with the SPSS 21 statistical software.
We found that () genotype polymorphism significantly was associated with increased susceptibility to TB (35% in patients vs. 22% in controls, P = 0.0417, OR = 1.909, %95 CI = 1.020-3.573). Additionally, allele showed a significant association with increased risk of TB (56.5% in patients vs. 46% in controls, P = 0.0357, OR = 1.525, %95 CI = 1.028-2.262). Also, the distribution of allele in patients was significantly lower frequency in TB patients compared to controls (43.5% vs. 54%, P = 0.0357, OR = 0.656, %95 CI = 0.442-0.973). However, the allelic and genotypic frequencies of , and polymorphisms were not significantly different between the patients and the controls. We couldn't detect any significant differences between haplotypes among TB patients and healthy controls.
Our findings demonstrated that genotype and allele may increase the susceptibility to pulmonary TB in the Lur population of Iran, although allele may decrease the susceptibility to pulmonary TB in this population. We suggest that it is necessary to further more studies with larger sample size and other ethnic population.
结核病（TB）由结核分枝杆菌感染引起。宿主基因变异性是人类患结核病风险的重要决定因素。尽管已在不同人群中研究了多态性与结核病之间的关联，但其结果存在争议。本研究旨在调查伊朗卢尔人群中甘露糖结合凝集素（MBL）基因多态性与肺结核（PTB）易感性的关系。
在这项病例对照研究中，采用聚合酶链反应单链构象多态性（PCR-SSCP）技术，对居住在洛雷斯坦省的100例肺结核患者和年龄、性别匹配的100名健康对照（HCs）组成的卢尔人群进行4种功能性MBL基因多态性（H/L、P/Q、X/Y和R/S）的基因分型。使用SPSS 21统计软件进行关联分析。
我们发现H/L（H/L）基因型多态性与结核病易感性增加显著相关（患者中为35%，对照中为22%，P = 0.0417，OR = 1.909，95%CI = 1.020 - 3.573）。此外，H等位基因与结核病风险增加显著相关（患者中为56.5%，对照中为46%，P = 0.0357，OR = 1.525，95%CI = 1.028 - 2.262）。而且，与对照相比，R等位基因在结核病患者中的分布频率显著较低（43.5%对54%，P = 0.0357，OR = 0.656，95%CI = 0.442 - 0.973）。然而，P/Q、X/Y和R/S多态性的等位基因和基因型频率在患者和对照之间无显著差异。我们未检测到结核病患者和健康对照之间单倍型有任何显著差异。
我们的研究结果表明，在伊朗卢尔人群中，H/L基因型和H等位基因可能增加肺结核易感性，尽管R等位基因可能降低该人群对肺结核的易感性。我们建议有必要用更大样本量和其他种族人群进行进一步研究。
