Shahsavar Farhad, Varzi Ali-Mohammad, Ahmadi Seyyed Amir Yasin
Department of immunology, Lorestan University of Medical Sciences, Khorramabad, Iran.
Research Office for the History of Persian Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran; Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran.
Genom Data. 2016 Nov 10;11:3-6. doi: 10.1016/j.gdata.2016.11.012. eCollection 2017 Mar.
Anthropological studies based on the highly polymorphic gene, (), provide useful information for bone marrow donor registry, forensic medicine, disease association studies, as well as infertility treatment, designing peptide vaccines against tumors, and infectious or autoimmune diseases. The aim of this study was to determine and allele frequencies in 100 unrelated Lak/lᴂk/individuals from Lorestan province of Iran. Finally, we compared the results with that previously described in Iranian population. Commercial HLA-Type kits from BAG (Lich, Germany) company were used for determination of the and allele frequencies in genomic DNA, based on polymerase chain reaction with sequence specific primer (PCR-SSP) assay. The differences between the populations in distribution of and alleles were estimated by chi-squared test with Yate's correction. The most frequent alleles were *24 (20%), *02 (18%), *03 (12%) and *11 (10%), and the most frequent alleles were *35 (24%), *51 (16%), *18 (6%) and *38 (6%) in Lak population. *66 (1%), *74(1%) and *48 (1%), *55(1%) were the least observed frequencies in Lak population. Our results based on and allele frequencies showed that Lak population possesses the previously reported general features of Iranians but still with unique.
基于高度多态性基因（）的人类学研究为骨髓供体登记、法医学、疾病关联研究以及不孕症治疗、设计针对肿瘤、感染性或自身免疫性疾病的肽疫苗提供了有用信息。本研究的目的是确定来自伊朗洛雷斯坦省的100名无关Lak/lᴂk/个体中的和等位基因频率。最后，我们将结果与先前在伊朗人群中描述的结果进行了比较。使用德国利希市BAG公司的商用HLA-Type试剂盒，基于序列特异性引物聚合酶链反应（PCR-SSP）分析来测定基因组DNA中的和等位基因频率。通过采用Yate校正的卡方检验估计人群中和等位基因分布的差异。在Lak人群中，最常见的等位基因是*24（20%）、02（18%）、03（12%）和11（10%），最常见的等位基因是35（24%）、*51（16%）、18（6%）和38（6%）。*66（1%）、74（1%）以及48（1%）、*55（1%）是Lak人群中观察到的频率最低的情况。我们基于和等位基因频率的结果表明，Lak人群具有先前报道的伊朗人的一般特征，但仍有独特之处。
