Yoon Hey Young, Hong Jong-In
Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 08826, Republic of Korea.
Data Brief. 2017 May 5;12:608-615. doi: 10.1016/j.dib.2017.04.056. eCollection 2017 Jun.
A new probe for detecting sulfatase activity generated fluorescent -methylisoindole when it was treated with sulfatase. Detailed synthetic procedures of reaction intermediates are described along with their spectral data. The Diels-Alder adduct of -methylisoindole and -phenylmaleimide was identified by LC-MS data. The fluorescence changes and kinetic values of the probe upon sulfatase treatment in the presence and absence of reducing agents were described. Inhibitory potency of EMATE was measured using the probe in the presence and absence of reducing agents. In addition, the fluorescence intensities of the probe upon sulfatase treatment were also monitored at pH 5 and 7 with and without reducing agents.
一种用于检测硫酸酯酶活性的新型探针，在与硫酸酯酶作用时会产生荧光的N-甲基异吲哚。文中描述了反应中间体的详细合成步骤及其光谱数据。通过液相色谱-质谱数据鉴定了N-甲基异吲哚与N-苯基马来酰亚胺的狄尔斯-阿尔德加合物。描述了在有和没有还原剂存在的情况下，该探针经硫酸酯酶处理后的荧光变化和动力学值。使用该探针在有和没有还原剂存在的情况下测定了EMATE的抑制效力。此外，还在pH 5和7条件下，有和没有还原剂存在的情况下监测了该探针经硫酸酯酶处理后的荧光强度。
