一组扩展的荧光硫酸酯酶活性探针。
An expanded set of fluorogenic sulfatase activity probes.
作者信息
Smith Elizabeth L, Bertozzi Carolyn R, Beatty Kimberly E
机构信息
Departments of Chemistry and Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, B84 Hildebrand Hall, #1460, Berkeley, CA 94720 (USA).
出版信息
Chembiochem. 2014 May 26;15(8):1101-5. doi: 10.1002/cbic.201400032. Epub 2014 Apr 24.
Abstract
Fluorogenic probes that are activated by an enzymatic transformation are ideally suited for profiling enzyme activities in biological systems. Here, we describe two fluorogenic enzyme probes, 3-O-methylfluorescein-sulfate and resorufin-sulfate, that can be used to detect sulfatases in mycobacterial lysates. Both probes were validated with a set of commercial sulfatases and used to reveal species-specific sulfatase banding patterns in a gel-resolved assay of mycobacterial lysates. The fluorogenic probes described here are suitable for various assays and provide a starting point for creating new sulfatase probes with improved selectivity for mycobacterial sulfatases.
摘要
通过酶促转化激活的荧光探针非常适合用于分析生物系统中的酶活性。在此，我们描述了两种荧光酶探针，硫酸3 - O - 甲基荧光素和硫酸试卤灵，它们可用于检测分枝杆菌裂解物中的硫酸酯酶。这两种探针均通过一组商业硫酸酯酶进行了验证，并用于在分枝杆菌裂解物的凝胶分辨分析中揭示物种特异性硫酸酯酶条带模式。本文所述的荧光探针适用于各种分析，并为创建对分枝杆菌硫酸酯酶具有更高选择性的新型硫酸酯酶探针提供了一个起点。
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