兔眼虹膜和睫状体中血小板活化因子的特异性受体位点。

Specific receptor sites for PAF in iris and ciliary body of the rabbit eye.

作者信息

van Delft J L, van Haeringen N J, Verbeij N L, Domingo M T, Chabrier P E, Braquet P

机构信息

Laboratory of the Department of Ophthalmology, University Hospital, Leiden, The Netherlands.

出版信息

Curr Eye Res. 1988 Nov;7(11):1063-8. doi: 10.3109/02713688809001876.

DOI:10.3109/02713688809001876

PMID:2854038

Abstract

The existence of specific binding sites by using tritiated 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine (3H-PAF) was investigated on iris and ciliary body tissue of pigmented rabbit eyes. The binding was saturable, specific, time-dependent and reversible. Scatchard's analysis indicated the presence of two types of binding sites with a Kd1 4.90 +/- 0.47 nM, a Kd2 11.60 +/- 0.33 nM, a Bmax1 3.17 +/- 0.50 pmol/mg protein and a Bmax2 12.45 +/- 2.30 pmol/mg protein for iris tissue, and a Kd1 5.71 +/- 0.09 nM, a K2d 24.40 +/- 0.91 nM, a Bmax1 3.41 +/- 1.00 pmol/mg protein and a Bmax2 16.60 +/- 0.51 pmol/mg protein for ciliary body. The binding was fully displaced by unlabelled PAF in both iris and ciliary body preparations, and partially inhibited by the PAF antagonist BN 52021 in iris tissue.

摘要

利用氚标记的1-O-烷基-2-O-乙酰基-sn-甘油-3-磷酸胆碱（3H-PAF），研究了色素兔眼虹膜和睫状体组织中特异性结合位点的存在情况。这种结合具有饱和性、特异性、时间依赖性和可逆性。Scatchard分析表明，虹膜组织存在两种类型的结合位点，其解离常数Kd1为4.90±0.47 nM，Kd2为11.60±0.33 nM，最大结合量Bmax1为3.17±0.50 pmol/mg蛋白，Bmax2为12.45±2.30 pmol/mg蛋白；睫状体的解离常数Kd1为5.71±0.09 nM，Kd2为24.40±0.91 nM，最大结合量Bmax1为3.41±1.00 pmol/mg蛋白，Bmax2为16.60±0.51 pmol/mg蛋白。在虹膜和睫状体标本中，未标记的PAF可完全取代这种结合，而PAF拮抗剂BN 52021可部分抑制虹膜组织中的结合。