Lalouette F, Diserbo M, Martin C, Verdetti J, Fatome M
Unité de Radioprotection Centre de Recherches du Service de Santé des Armées Emile Pardé 24, La Tronche, France.
Neurosci Lett. 1995 Feb 17;186(2-3):173-6. doi: 10.1016/0304-3940(95)11319-r.
In this study we reported evidence for the existence of specific binding sites for platelet-activating factor (PAF) in neuroblastoma N1E-115 cells. The specific [3H]PAF binding reached a steady state level within 60 min at 25 degrees C. Scatchard analysis of the specific [3H]PAF binding revealed the presence of two apparent populations of binding sites. The high-affinity binding site possessed a Kd1 of 2.5 +/- 0.6 pM and Bmax1 = 57.3 +/- 20.0 fmol/mg protein. The low-affinity binding site possessed a Kd2 = 3.2 +/- 1.0 nM and Bmax2 = 4.4 +/- 2.1 pmol/mg protein. Furthermore, the total [3H]PAF binding was partially displaced by unlabelled PAF, PAF antagonists BN 52021 and BN 50730 in a dose-dependent manner. This study confirms the presence of specific PAF receptors in neuronal cells.
在本研究中，我们报告了神经母细胞瘤N1E - 115细胞中存在血小板活化因子（PAF）特异性结合位点的证据。在25℃下，特异性[³H]PAF结合在60分钟内达到稳态水平。对特异性[³H]PAF结合的Scatchard分析显示存在两种明显的结合位点群体。高亲和力结合位点的Kd1为2.5±0.6 pM，Bmax1 = 57.3±20.0 fmol/mg蛋白质。低亲和力结合位点的Kd2 = 3.2±1.0 nM，Bmax2 = 4.4±2.1 pmol/mg蛋白质。此外，未标记的PAF、PAF拮抗剂BN 52021和BN 50730以剂量依赖性方式部分取代了总的[³H]PAF结合。本研究证实了神经元细胞中存在特异性PAF受体。
