Lambeth J D
Department of Biochemistry, Emory University Medical School, Atlanta, Georgia 30322.
J Bioenerg Biomembr. 1988 Dec;20(6):709-33. doi: 10.1007/BF00762549.
A major bactericidal mechanism of neutrophils involves activation of the respiratory burst oxidase to generate superoxide (O2-). The oxidase is activated rapidly, often within a minute, in response to extracellular signals such as chemoattractants, inflammatory mediators, and invading microorganisms. Increasing evidence indicates that lipases also respond rapidly, releasing potent regulatory molecules from progenitor lipids. Released molecules include potential regulators of protein kinase C--diacylglycerol (DAG), arachidonate, and sphingosine--and levels of one of these, DAG, frequently correlate with O2- production. In this author's view, the available data implicate DAG and protein kinase C as key factors in the regulation of the respiratory burst. Herein, the array of activating agonists, the generation and function of some lipid-derived mediators, and evidence pertaining to the participation of protein kinase C are reviewed.
中性粒细胞的一种主要杀菌机制涉及呼吸爆发氧化酶的激活，以产生超氧化物（O2-）。氧化酶会迅速被激活，通常在一分钟内，以响应细胞外信号，如趋化因子、炎症介质和入侵微生物。越来越多的证据表明，脂肪酶也会迅速做出反应，从祖脂中释放出强效调节分子。释放的分子包括蛋白激酶C的潜在调节剂——二酰基甘油（DAG）、花生四烯酸和鞘氨醇——其中之一DAG的水平通常与O2-的产生相关。在作者看来，现有数据表明DAG和蛋白激酶C是呼吸爆发调节中的关键因素。本文综述了一系列激活激动剂、一些脂质衍生介质的产生和功能，以及与蛋白激酶C参与相关的证据。
