Chen Lulu, Cheng Jia, Yang Xiaonan, Jin Xiaolei, Qi Zuoliang, Jin Yu-Qing
Department No.16 of Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Department of Burn and Plastic Surgery, Wuxi 3rd People's Hospital, Wuxi, Jiangsu, China.
Cell Biol Int. 2017 Aug;41(8):863-870. doi: 10.1002/cbin.10796. Epub 2017 Jun 13.
In recent years, bone marrow-derived cells have been found to be crucial for peripheral nerve regeneration. After traumatic peripheral nerve injury, bone marrow-derived macrophages quickly infiltrate into the distal regions of nerves. To explore the changes caused by bone marrow-derived cells within the proximal regions of the nerves, sciatic nerves of chimeric mice carrying bone marrow cells expressing green fluorescent protein were crushed to observe the infiltration of invading bone marrow-derived cells. Seven days after surgery, abundant bone marrow-derived cells had infiltrated into the damaged proximal nerve segments. The numbers of these cells increased to a peak at 2 weeks and then gradually returned to normal levels within 30 weeks. Through immunofluorescence staining, many of these cells were identified as macrophages, and they showed a similar infiltration tendency toward distal nerve segments. However, fewer cells infiltrated proximal segments than distal nerve segments. In conclusion, these findings suggest that bone marrow-derived cells response not only occurs within the distal nerve segments but may also take place within the proximal segments of nerve tissues after nerve injury.
近年来，人们发现骨髓来源的细胞对周围神经再生至关重要。外伤性周围神经损伤后，骨髓来源的巨噬细胞会迅速浸润到神经的远端区域。为了探究骨髓来源的细胞在神经近端区域所引起的变化，对携带表达绿色荧光蛋白的骨髓细胞的嵌合小鼠的坐骨神经进行挤压，以观察侵入的骨髓来源细胞的浸润情况。术后7天，大量骨髓来源的细胞浸润到受损的近端神经节段。这些细胞的数量在2周时增加到峰值，然后在30周内逐渐恢复到正常水平。通过免疫荧光染色，其中许多细胞被鉴定为巨噬细胞，并且它们对远端神经节段表现出相似的浸润趋势。然而，浸润到近端节段的细胞比远端神经节段的少。总之，这些发现表明，骨髓来源的细胞反应不仅发生在远端神经节段，在神经损伤后神经组织的近端节段也可能发生。
