Course of Physics, Graduate School of Science, Tokai University, Hiratsuka City, Kanagawa, Japan.
Department of Physical Therapy, Human Health Sciences, Graduate school of Medicine, Kyoto University, Kyoto City, Kyoto, Japan.
PLoS One. 2021 Jun 2;16(6):e0252589. doi: 10.1371/journal.pone.0252589. eCollection 2021.
Electrical stimulation is one of the candidates for elongation-driven regeneration of damaged peripheral nerves. Different organs and tissues have an inherent cell structure and size. This leads to variation in the tissue-specific electrical properties of the frequency of interfacial polarization. Although nervous tissues have a membrane potential, the electrical reaction inside these tissues following electrical stimulation from outside remains unexplored. Furthermore, the pathophysiological reaction of an injured nerve is unclear. Here, we investigated the electrical reaction of injured and non-injured rat sciatic nerves via broadband dielectric spectroscopy. Crush injured and non-injured sciatic nerves of six 12-week-old male Lewis rats were used, 6 days after infliction of the injury. Both sides of the nerves (with and without injury) were exposed, and impedance measurements were performed at room temperature (approximately 25°C) at frequencies ranging from 100 mHz to 5.5 MHz and electric potential ranging from 0.100 to 1.00 V. The measured interfacial polarization potentially originated from the polarization by ion transport around nerve membranes at frequencies between 3.2 kHz and 1.6 MHz. The polarization strength of the injured nerves was smaller than that of non-injured nerves. However, the difference in polarization between injured and non-injured nerves might be caused by inflammation and edema. The suitable frequency range of the interfacial polarization can be expected to be critical for electrical stimulation of injured peripheral nerves.
电刺激是一种用于损伤外周神经的伸长驱动再生的候选方法。不同的器官和组织具有固有细胞结构和大小。这导致界面极化频率的组织特异性电特性的变化。尽管神经组织具有膜电位，但外部电刺激后这些组织内的电反应仍未被探索。此外，损伤神经的病理生理反应尚不清楚。在这里，我们通过宽带介电谱研究了损伤和未损伤大鼠坐骨神经的电反应。使用 6 只 12 周龄雄性 Lewis 大鼠的挤压损伤和未损伤的坐骨神经，在损伤后 6 天。暴露神经的两侧（有和没有损伤），并在室温（约 25°C）下在 100 mHz 至 5.5 MHz 的频率和 0.100 至 1.00 V 的电势下进行阻抗测量。测量的界面极化可能源自神经膜周围离子迁移的极化，频率在 3.2 kHz 和 1.6 MHz 之间。损伤神经的极化强度小于未损伤神经的极化强度。然而，损伤和未损伤神经之间的极化差异可能是由炎症和水肿引起的。界面极化的合适频率范围有望成为损伤外周神经电刺激的关键。
