State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, China.
J Integr Plant Biol. 2017 Sep;59(9):594-599. doi: 10.1111/jipb.12556. Epub 2017 Jun 21.
Pollen development is a pre-requisite for sexual reproduction of angiosperms, during which various cellular activities are involved. Pollen development accompanies dynamic remodeling of vacuoles through fission and fusion, disruption of which often compromises pollen viability. We previously reported that the Y subunit of adaptor protein 1 (AP1G) mediates synergid degeneration during pollen tube reception. Here, we demonstrate that AP1G is essential for pollen development. AP1G loss-of-function resulted in male gametophytic lethality due to defective pollen development. By ultrastructural analysis and fluorescence labeling, we demonstrate that AP1G loss-of-function compromised dynamic vacuolar remodeling during pollen development and impaired vacuolar acidification of pollen. Results presented here support a key role of vacuoles in gametophytic pollen development.
花粉发育是被子植物有性生殖的前提，在此过程中涉及到各种细胞活动。花粉发育伴随着液泡的分裂和融合的动态重塑，其破坏常常会影响花粉的活力。我们之前报道了衔接蛋白 1（AP1G）的 Y 亚基在花粉管接收过程中介导助细胞退化。在这里，我们证明了 AP1G 对花粉发育是必需的。AP1G 功能丧失导致雄性配子体致死，因为花粉发育缺陷。通过超微结构分析和荧光标记，我们证明了 AP1G 功能丧失会损害花粉发育过程中动态液泡重塑，并损害花粉的液泡酸化。这里呈现的结果支持液泡在配子体花粉发育中的关键作用。
