Xu Ling, Wu Hong, Wu Xiuli, Li Yangqiu, He Dongmei
Institute of Hematology, Medical College, Jinan University, Guangzhou, PR China.
Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, PR China.
Asia Pac J Clin Oncol. 2018 Apr;14(2):e124-e128. doi: 10.1111/ajco.12690. Epub 2017 May 24.
Bcl11a is closely associated with B-cell lymphoma and B-cell chronic lymphocytic leukemia. However, little is known about the expression character of Bcl11a gene in B-cell acute lymphoblastic leukemia (B-ALL). In previous study, our results showed there was a clear dysregulation in the global gene expression of the Bcl11a-suppressed B lymphoma cells. The aim of the study is to further evaluate the role of Bcl11a and the expression level of its related genes Mdm2, Pten in B-ALL patients.
The relative mRNA expression levels of Bcl11a and Mdm2, Pten in blood mononuclear cells of B-ALL patients were determined by real-time quantitative reverse transcript-polymerase chain reaction (qRT-PCR) with SYBR Green Dye. Peripheral blood mononuclear cells from healthy volunteers served as control. Glyceraldehyde-3-phosphate dehydrogenase (Gapdh) was used as reference.
There was higher Bcl11a mRNA expression in initial diagnosed B-ALL than the healthy control, but there is no statistical significance (P > 0.05). The expression level of Bcl11a and Mdm2 in initial diagnosed B-ALL patients was statistically higher compared to those with complete remission (CR; P < 0.05). The expression levels of Bcl11a and Mdm2, Pten in B-ALL patients with CR were decreased significantly when compared with the healthy control (P < 0.05). The correlation analyses revealed that the Bcl11a expression level was positively correlated with the Mdm2 and Pten relative expression level in B-ALL patients. There is also positive correlation between Mdm2 and Pten expression.
Bcl11a transcript levels were significantly downregulated in B-ALL patients with CR. The decreased expression characteristics of Bcl11a and Mdm2, Pten might imply the CR of B-ALL patients.
Bcl11a与B细胞淋巴瘤及B细胞慢性淋巴细胞白血病密切相关。然而，关于Bcl11a基因在B细胞急性淋巴细胞白血病（B-ALL）中的表达特征知之甚少。在先前的研究中，我们的结果显示Bcl11a抑制的B淋巴瘤细胞的整体基因表达存在明显失调。本研究的目的是进一步评估Bcl11a及其相关基因Mdm2、Pten在B-ALL患者中的作用。
采用SYBR Green染料实时定量逆转录聚合酶链反应（qRT-PCR）测定B-ALL患者血液单核细胞中Bcl11a、Mdm2和Pten的相对mRNA表达水平。来自健康志愿者的外周血单核细胞作为对照。甘油醛-3-磷酸脱氢酶（Gapdh）用作参照。
初诊B-ALL患者的Bcl11a mRNA表达高于健康对照，但无统计学意义（P>0.05）。初诊B-ALL患者中Bcl11a和Mdm2的表达水平与完全缓解（CR）患者相比有统计学意义的升高（P<0.05）。与健康对照相比，CR的B-ALL患者中Bcl11a、Mdm2和Pten的表达水平显著降低（P<0.05）。相关性分析显示，B-ALL患者中Bcl11a表达水平与Mdm2和Pten相对表达水平呈正相关。Mdm2与Pten表达之间也呈正相关。
CR的B-ALL患者中Bcl11a转录水平显著下调。Bcl11a、Mdm2和Pten表达降低的特征可能意味着B-ALL患者的CR。
