• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

促进神经母细胞瘤的细胞增殖和转移，通过调节 PI3K/Akt 信号通路。

Facilitates Cell Proliferation and Metastasis in Neuroblastoma via Regulating the PI3K/Akt Signaling Pathway.

机构信息

Department of Pediatric Hematology/Oncology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.

Department of Medicine, Quzhou College of Technology, Quzhou, Zhejiang Province 324000, China.

出版信息

Curr Cancer Drug Targets. 2022;22(11):919-930. doi: 10.2174/1568009622666220728123748.

DOI:10.2174/1568009622666220728123748
PMID:35909289
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9900700/
Abstract

PURPOSE

The study aims to access the value of B-cell lymphoma/leukemia 11A (BCL11A) in the prognosis of patients with neuroblastoma (NB) and to explore its role and possible mechanism in NB.

METHODS

Tumor specimens from 53 children with neuroblastoma were evaluated for the relationship between BCL11A expression level and prognosis of NB patients. Online datasets like SEQC and Asgharzadeh were analyzed to further check out the suppose.The role of BCL11A in the proliferation and migration of NB cells was studied by functional experiments such as CCK8, colony formation, flow cytometry, transwell and wound healing assay after knocking down BCL11A by small interfering RNA (siRNA) in vitro. The protein makers of the potential pathways were tested by western blot.

RESULTS

High expression of BCL11A in NB patients was closely correlated with high-risk and poor prognosis. The proliferation and migration abilities of NB cell lines SK-N-BE(2) and IMR-32 were significantly impaired by silencing BCL11A. Downregulation of BCL11A expression level in NB cells inhibited the epithelial-mesenchymal transition (EMT) process and affected the PI3K/Akt signaling pathway.

CONCLUSION

As a prognostic indicator of survival in NB patients, BCL11A might serve as a potential therapeutic target. BCL11A played a regulatory role in cell proliferation, invasion, and migration in NB, which may be through the PI3K/AKT signaling pathway and induce EMT.

摘要

目的

本研究旨在评估 B 细胞淋巴瘤/白血病 11A（BCL11A）在神经母细胞瘤（NB）患者预后中的价值，并探讨其在 NB 中的作用及可能的机制。

方法

对 53 例神经母细胞瘤患儿的肿瘤标本进行评估，分析 BCL11A 表达水平与 NB 患者预后的关系。在线数据集如 SEQC 和 Asgharzadeh 进行分析以进一步验证假设。通过体外小干扰 RNA（siRNA）敲低 BCL11A 研究 BCL11A 对 NB 细胞增殖和迁移的作用，采用 CCK8、集落形成、流式细胞术、Transwell 和划痕愈合实验。通过 Western blot 检测潜在通路的蛋白标志物。

结果

NB 患者中 BCL11A 的高表达与高危和预后不良密切相关。沉默 BCL11A 可显著抑制 NB 细胞系 SK-N-BE(2)和 IMR-32 的增殖和迁移能力。NB 细胞中 BCL11A 表达水平的下调抑制了上皮间质转化（EMT）过程，并影响了 PI3K/Akt 信号通路。

结论

BCL11A 作为 NB 患者生存的预后指标，可能成为潜在的治疗靶点。BCL11A 在 NB 中对细胞增殖、侵袭和迁移起调节作用，可能通过 PI3K/AKT 信号通路诱导 EMT。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/8757bd90095b/CCDT-22-919_F6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/b09e02458cd2/CCDT-22-919_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/ff4bd6fc43d0/CCDT-22-919_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/3b59d722288b/CCDT-22-919_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/83ae2df7b67d/CCDT-22-919_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/5a0733864a7e/CCDT-22-919_F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/8757bd90095b/CCDT-22-919_F6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/b09e02458cd2/CCDT-22-919_F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/ff4bd6fc43d0/CCDT-22-919_F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/3b59d722288b/CCDT-22-919_F3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/83ae2df7b67d/CCDT-22-919_F4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/5a0733864a7e/CCDT-22-919_F5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b11/9900700/8757bd90095b/CCDT-22-919_F6.jpg

相似文献

1
Facilitates Cell Proliferation and Metastasis in Neuroblastoma via Regulating the PI3K/Akt Signaling Pathway.促进神经母细胞瘤的细胞增殖和转移，通过调节 PI3K/Akt 信号通路。
Curr Cancer Drug Targets. 2022;22(11):919-930. doi: 10.2174/1568009622666220728123748.
2
RNA-seq analysis reveals candidate genes associated with proliferation, invasion, and migration in BCL11A knockdown B-NHL cell lines.RNA-seq 分析揭示了与 BCL11A 敲低 B-NHL 细胞系增殖、侵袭和迁移相关的候选基因。
Ann Hematol. 2023 Jul;102(7):1845-1856. doi: 10.1007/s00277-023-05247-w. Epub 2023 May 6.
3
TEAD4 functions as a prognostic biomarker and triggers EMT via PI3K/AKT pathway in bladder cancer.TEAD4 在膀胱癌中作为预后生物标志物，通过 PI3K/AKT 通路触发 EMT。
J Exp Clin Cancer Res. 2022 May 17;41(1):175. doi: 10.1186/s13046-022-02377-3.
4
Intelectin 1 suppresses the growth, invasion and metastasis of neuroblastoma cells through up-regulation of N-myc downstream regulated gene 2.肝胰凝集素1通过上调N - myc下游调控基因2抑制神经母细胞瘤细胞的生长、侵袭和转移。
Mol Cancer. 2015 Feb 21;14:47. doi: 10.1186/s12943-015-0320-6.
5
ISL1 promoted tumorigenesis and EMT via Aurora kinase A-induced activation of PI3K/AKT signaling pathway in neuroblastoma.ISL1 通过激活 Aurora 激酶 A 诱导的 PI3K/AKT 信号通路促进神经母细胞瘤的肿瘤发生和 EMT。
Cell Death Dis. 2021 Jun 15;12(6):620. doi: 10.1038/s41419-021-03894-3.
6
FoxM1 Regulates Proliferation and Apoptosis of Human Neuroblastoma Cell through PI3K/AKT Pathway.FoxM1 通过 PI3K/AKT 通路调节人神经母细胞瘤细胞的增殖和凋亡。
Fetal Pediatr Pathol. 2022 Jun;41(3):355-370. doi: 10.1080/15513815.2020.1814915. Epub 2020 Sep 9.
7
[Role and mechanism of cysteine and glycine-rich protein 2 in the malignant progression of neuroblastoma].富含半胱氨酸和甘氨酸蛋白2在神经母细胞瘤恶性进展中的作用及机制
Beijing Da Xue Xue Bao Yi Xue Ban. 2024 Jun 18;56(3):495-504. doi: 10.19723/j.issn.1671-167X.2024.03.017.
8
Microrchidia family CW‑type zinc finger 2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of glioma by regulating PTEN/PI3K/AKT signaling via binding to N‑myc downstream regulated gene 1 promoter.微线体家族 CW 型锌指蛋白 2 通过结合 N‑myc 下游调节基因 1 启动子调控 PTEN/PI3K/AKT 信号通路，促进胶质瘤的增殖、侵袭、迁移和上皮间质转化。
Int J Mol Med. 2022 Feb;49(2). doi: 10.3892/ijmm.2021.5071. Epub 2021 Dec 16.
9
Polo-like kinase 4 mediates epithelial-mesenchymal transition in neuroblastoma via PI3K/Akt signaling pathway.Polo-like kinase 4 通过 PI3K/Akt 信号通路介导神经母细胞瘤中的上皮-间充质转化。
Cell Death Dis. 2018 Jan 19;9(2):54. doi: 10.1038/s41419-017-0088-2.
10
Oncogenic gene RGC-32 is a direct target of miR-26b and facilitates tongue squamous cell carcinoma aggressiveness through EMT and PI3K/AKT signalling.致癌基因 RGC-32 是 miR-26b 的直接靶标，通过 EMT 和 PI3K/AKT 信号通路促进舌鳞状细胞癌的侵袭性。
Cell Biochem Funct. 2020 Oct;38(7):943-954. doi: 10.1002/cbf.3520. Epub 2020 Apr 23.

引用本文的文献

1
Study of an N6-methyladenosine- and ferroptosis-related prognostic model and the mechanisms underlying the molecular network in neuroblastoma based on multiple datasets.基于多个数据集的神经母细胞瘤中N6-甲基腺苷与铁死亡相关预后模型及分子网络潜在机制的研究
Discov Oncol. 2025 Feb 18;16(1):200. doi: 10.1007/s12672-025-01975-9.
2
Combining network pharmacology and RNA sequencing to reveal the mechanism of emodin for the treatment of human neuroblastoma.结合网络药理学和RNA测序揭示大黄素治疗人类神经母细胞瘤的机制。
Naunyn Schmiedebergs Arch Pharmacol. 2025 Feb 13. doi: 10.1007/s00210-025-03865-x.
3
The glucocorticoid receptor elicited proliferative response in human erythropoiesis is BCL11A-dependent.

本文引用的文献

1
MYCN in Neuroblastoma: "Old Wine into New Wineskins".神经母细胞瘤中的MYCN：“旧瓶装新酒”
Diseases. 2021 Oct 29;9(4):78. doi: 10.3390/diseases9040078.
2
Molecular targeting therapies for neuroblastoma: Progress and challenges.神经母细胞瘤的分子靶向治疗：进展与挑战。
Med Res Rev. 2021 Mar;41(2):961-1021. doi: 10.1002/med.21750. Epub 2020 Nov 6.
3
BCL11A Is Oncogenic and Predicts Poor Outcomes in Natural Killer/T-Cell Lymphoma.BCL11A具有致癌性，并预示自然杀伤/T细胞淋巴瘤的预后不良。
糖皮质激素受体在人类红细胞生成中引发的增殖反应依赖于 BCL11A。
Stem Cells. 2024 Nov 5;42(11):1006-1022. doi: 10.1093/stmcls/sxae049.
4
ADGRL4 Promotes Cell Growth, Aggressiveness, EMT, and Angiogenesis in Neuroblastoma via Activation of ERK/STAT3 Pathway.ADGRL4通过激活ERK/STAT3通路促进神经母细胞瘤的细胞生长、侵袭性、上皮-间质转化和血管生成。
Curr Mol Med. 2025;25(1):45-55. doi: 10.2174/0115665240254765231117122210.
Front Pharmacol. 2020 Jun 4;11:820. doi: 10.3389/fphar.2020.00820. eCollection 2020.
4
BCL11A Promotes the Progression of Laryngeal Squamous Cell Carcinoma.BCL11A促进喉鳞状细胞癌的进展。
Front Oncol. 2020 Mar 20;10:375. doi: 10.3389/fonc.2020.00375. eCollection 2020.
5
BCL11A confers cell invasion and migration in androgen receptor-positive triple-negative breast cancer.BCL11A赋予雄激素受体阳性三阴性乳腺癌细胞侵袭和迁移能力。
Oncol Lett. 2020 Apr;19(4):2916-2924. doi: 10.3892/ol.2020.11383. Epub 2020 Feb 10.
6
BCL11A gene over-expression in high risk neuroblastoma.高危神经母细胞瘤中BCL11A基因的过表达
Cancer Genet. 2020 Jun;244:30-31. doi: 10.1016/j.cancergen.2020.02.003. Epub 2020 Feb 27.
7
BCL11A: a potential diagnostic biomarker and therapeutic target in human diseases.BCL11A：人类疾病潜在的诊断生物标志物和治疗靶点。
Biosci Rep. 2019 Nov 29;39(11). doi: 10.1042/BSR20190604.
8
BCL11A enhances stemness and promotes progression by activating Wnt/β-catenin signaling in breast cancer.BCL11A通过激活乳腺癌中的Wnt/β-连环蛋白信号增强干性并促进进展。
Cancer Manag Res. 2019 Apr 11;11:2997-3007. doi: 10.2147/CMAR.S199368. eCollection 2019.
9
Current management of neuroblastoma and future direction.神经母细胞瘤的当前治疗方法和未来方向。
Crit Rev Oncol Hematol. 2019 Jun;138:38-43. doi: 10.1016/j.critrevonc.2019.03.013. Epub 2019 Apr 1.
10
BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma.BCL11A 与 SOX2 相互作用，以控制肺鳞癌中表观遗传调节剂的表达。
Nat Commun. 2018 Aug 20;9(1):3327. doi: 10.1038/s41467-018-05790-5.