Department of Entomology, China Agricultural University, Beijing, China.
Pest Manag Sci. 2017 Nov;73(11):2353-2359. doi: 10.1002/ps.4622. Epub 2017 Aug 2.
The cotton aphid, Aphis gossypii (Glover), is a destructive pest that affects numerous crops throughout the world. Pyrethroid resistance has become endemic in A. gossypii populations in the cotton growing areas of China due to wide- spread application of insecticides. To assess the extent of pyrethroid resistance, bioassays were conducted on field populations collected from several cotton cultivation areas from 2010 to 2015. The frequency of a known resistance-associated sodium channel mutation (M918 L) in A. gossypii was evaluated and the bioassay of bifenthrin with or without the synergist was performed to illuminate the mechanisms underlying resistance to pyrethroids.
The field populations exhibited very high levels of resistance to both beta-cypermethrin and deltamethrin. Pretreatment with synergists, DEF and PBO, significantly increased the toxicity of bifenthrin to cotton aphid populations collected from Bt cotton fields in China. Further, 96.8-100% of individuals with the M918 L mutation (including both RR and RS individuals) were observed in various populations, and only 2.8-3.2% of individuals with wild-type homozygotes (SS) were detected.
The mutation M918 L in the voltage-gated sodium channel along with detoxifying metabolism was contributed to the pyrethroid resistance in the field populations of Aphis gossypii from cotton growing regions of China. And insecticides with different modes of action should be recommended for the control of A. gossypii in the future. © 2017 Society of Chemical Industry.
棉蚜，Aphis gossypii（Glover），是一种破坏性害虫，影响世界上许多作物。由于杀虫剂的广泛应用，在中国的棉花种植区，A. gossypii 种群中已出现拟除虫菊酯抗性。为了评估拟除虫菊酯抗性的程度，我们于 2010 年至 2015 年对从多个棉花种植区采集的田间种群进行了生物测定。评估了 A. gossypii 中已知的与抗性相关的钠离子通道突变（M918L）的频率，并进行了含有或不含有增效剂的联苯菊酯的生物测定，以阐明对拟除虫菊酯产生抗性的机制。
田间种群对溴氰菊酯和氯氰菊酯均表现出极高的抗性。用增效剂 DEF 和 PBO 预处理可显著提高中国转 Bt 棉田棉蚜种群对联苯菊酯的毒性。此外，在各种种群中观察到 96.8-100%的个体具有 M918L 突变（包括 RR 和 RS 个体），而仅检测到 2.8-3.2%的野生型纯合子（SS）个体。
电压门控钠离子通道中的突变 M918L 与解毒代谢一起导致了来自中国棉花种植区的棉蚜田间种群对拟除虫菊酯的抗性。未来应推荐使用具有不同作用模式的杀虫剂来防治 A. gossypii。© 2017 化学工业协会。
