Cai Yuhao, Chen Huilin, Hu Mengfan, Wang Xuegui, Zhang Lei
Department of Entomology, China Agricultural University, Beijing 100193, China.
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
Insects. 2024 Mar 11;15(3):186. doi: 10.3390/insects15030186.
The fall armyworm (FAW), , is a worldwide agricultural pest that invaded China in 2018, and has developed resistance to multiple insecticides. The evolution of insecticide resistance is facilitated by mutations of target genes responsible for conferring resistance. In this study, amplicon sequencing analyzed 21 sites in six resistance genes. In addition to known mutations, unknown variants were also found, including novel variants: F290C ( gene, 0.1% frequency), I1040T/V ( gene, 0.1% frequency), A309T ( gene, 0.1% frequency), and I4790T/V ( gene, 0.1% frequency). Additionally, molecular docking was employed to investigate the impact of the aforementioned new mutations on insecticide binding to proteins. The analyses indicated that the binding abilities were reduced, similar to the resistance mutations that were reported, implying these novel mutations may confer transitional resistance. This study may provide a foundation for understanding the functions of these novel mutations in the evolutionary processes that drive the emergence of insecticide resistance in this invasive species.
草地贪夜蛾是一种世界性农业害虫，于2018年入侵中国，并且已对多种杀虫剂产生抗性。负责赋予抗性的靶基因突变促进了杀虫剂抗性的进化。在本研究中，扩增子测序分析了6个抗性基因中的21个位点。除了已知突变外，还发现了未知变体，包括新变体：F290C（基因，频率0.1%）、I1040T/V（基因，频率0.1%）、A309T（基因，频率0.1%）和I4790T/V（基因，频率0.1%）。此外，采用分子对接来研究上述新突变对杀虫剂与蛋白质结合的影响。分析表明，结合能力降低，类似于已报道的抗性突变，这意味着这些新突变可能赋予过渡性抗性。本研究可为理解这些新突变在驱动该入侵物种产生杀虫剂抗性的进化过程中的功能提供基础。
