Wang Ran, Zhang Kaiyue, Tao Hongyan, Du Wei, Wang Di, Huang Ziwei, Zhou Manqian, Xu Yang, Wang Yuebing, Liu Na, Wang Hui, Li Zongjin
Nankai University School of Medicine, Tianjin 300071, China.
Department of Radiation Oncology, Tianjin Union Medical Center, Tianjin 300121, China.
Curr Pharm Biotechnol. 2017;18(5):422-428. doi: 10.2174/1389201018666170523165053.
Angiogenesis is critical for the growth of tumor by supplying nutrients and oxygen that exacerbates the metastasis and progression of cancer. Noninvasive imaging of angiogenesis during the tumor therapeutic processes may provide novel opportunities for image-guided tumor management.
Here, we want to develop a mouse animal model for assessing cancer progression and angiogenesis in the same individuals by molecular imaging.
Breast cancer model was developed with mouse breast cancer cell line 4T1 carrying a reporter system encoding a triple fusion (TF) reporter gene consisting of renilla luciferase (Rluc), red fluorescent protein (RFP) and herpes simplex virus truncated thymidine kinase (HSV-ttk) in transgenic mice, which expressed firefly luciferase (Fluc) under the promoter of vascular endothelial growth factor receptor 2 (Vegfr2-luc). The mice were subsequently treated with ganciclovir (GCV) and the tumor angiogenesis was tracked by Fluc imaging and the growth status of tumor was monitored by imaging of Rluc simultaneously.
Overall, this traceable breast cancer model can simultaneously image the tumor growth and angiogenesis in single individual, which may facilitate a better understanding the mechanisms of angiogenesis in the progression and regression of tumor.
血管生成对于肿瘤生长至关重要，它通过提供营养物质和氧气来加剧癌症的转移和进展。在肿瘤治疗过程中对血管生成进行无创成像可能为图像引导的肿瘤管理提供新的机会。
在此，我们希望通过分子成像开发一种小鼠动物模型，用于评估同一个体中的癌症进展和血管生成。
利用携带由海肾荧光素酶（Rluc）、红色荧光蛋白（RFP）和单纯疱疹病毒截短胸苷激酶（HSV-ttk）组成的三融合（TF）报告基因的报告系统的小鼠乳腺癌细胞系4T1，在转基因小鼠中建立乳腺癌模型，这些小鼠在血管内皮生长因子受体2（Vegfr2-luc）启动子下表达萤火虫荧光素酶（Fluc）。随后用更昔洛韦（GCV）处理小鼠，并通过Fluc成像追踪肿瘤血管生成，同时通过Rluc成像监测肿瘤生长状态。
总体而言，这种可追踪的乳腺癌模型能够在单个个体中同时对肿瘤生长和血管生成进行成像，这可能有助于更好地理解肿瘤进展和消退过程中血管生成的机制。
