大象促肾上腺皮质激素和β-促脂素的分离及一级结构
Isolation and primary structures of elephant adrenocorticotropin and beta-lipotropin.
作者信息
Li C H, Oosthuizen M M, Chung D
机构信息
Laboratory of Molecular Endocrinology, University of California, San Francisco.
出版信息
Int J Pept Protein Res. 1988 Dec;32(6):573-8. doi: 10.1111/j.1399-3011.1988.tb01389.x.
Abstract
Adrenocorticotropin and beta-lipotropin have been isolated and purified from elephant pituitary glands. The primary structures of these two hormones were determined by amino acid and sequence analyses of enzymatically cleaved peptides from the hormones. Peptide purification involved the use of gel filtration, reverse phase high performance liquid chromatography, and paper electrophoresis.
摘要
促肾上腺皮质激素和β-促脂素已从大象垂体中分离并纯化出来。通过对这些激素经酶切产生的肽段进行氨基酸分析和序列分析，确定了这两种激素的一级结构。肽段的纯化涉及凝胶过滤、反相高效液相色谱和纸电泳等方法。
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