Scott A P, Lowry P J
Biochem J. 1974 Jun;139(3):593-602. doi: 10.1042/bj1390593.
The adrenocorticotrophic and melanocyte-stimulating peptides of the human pituitary were investigated by means of radioimmunoassay, bioassay and physicochemical procedures. Substantial amounts of adrenocorticotrophin and a peptide resembling beta-lipotrophin were identified in pituitary extracts, but alpha-melanocyte-stimulating hormone, beta-melanocyte-stimulating hormone and corticotrophin-like intermediate lobe peptide, which have been identified in the pars intermedia of pituitaries from other vertebrates, were not found. The absence of beta-melanocyte-stimulating hormone appears to contradict previous chemical and radioimmunological studies. Our results suggest, however, that it is not a natural pituitary peptide but an artefact formed by enzymic degradation of beta-lipotrophin during extraction.
通过放射免疫测定、生物测定和物理化学方法对人垂体的促肾上腺皮质激素和促黑素细胞肽进行了研究。在垂体提取物中鉴定出了大量的促肾上腺皮质激素和一种类似于β-促脂解素的肽，但未发现α-促黑素细胞激素、β-促黑素细胞激素和促肾上腺皮质激素样中间叶肽，而这些物质在其他脊椎动物垂体的中间部已被鉴定出来。β-促黑素细胞激素的缺失似乎与先前的化学和放射免疫研究结果相矛盾。然而，我们的结果表明，它不是一种天然的垂体肽，而是在提取过程中由β-促脂解素酶解形成的一种人工产物。
