Goodman Daniel B, Kuznetsov Gleb, Lajoie Marc J, Ahern Brian W, Napolitano Michael G, Chen Kevin Y, Chen Changping, Church George M
Department of Genetics, Harvard Medical School, Boston, MA, USA.
Wyss Institute for Biologically Inspired Engineering, Harvard Medical School, Boston, MA, USA.
Genome Biol. 2017 May 25;18(1):101. doi: 10.1186/s13059-017-1223-1.
Inexpensive DNA sequencing and advances in genome editing have made computational analysis a major rate-limiting step in adaptive laboratory evolution and microbial genome engineering. We describe Millstone, a web-based platform that automates genotype comparison and visualization for projects with up to hundreds of genomic samples. To enable iterative genome engineering, Millstone allows users to design oligonucleotide libraries and create successive versions of reference genomes. Millstone is open source and easily deployable to a cloud platform, local cluster, or desktop, making it a scalable solution for any lab.
廉价的DNA测序技术和基因组编辑技术的进步，使得计算分析成为适应性实验室进化和微生物基因组工程中的主要限速步骤。我们介绍了Millstone，这是一个基于网络的平台，可对多达数百个基因组样本的项目进行基因型比较和可视化的自动化操作。为实现迭代基因组工程，Millstone允许用户设计寡核苷酸文库并创建参考基因组的连续版本。Millstone是开源的，可轻松部署到云平台、本地集群或桌面，使其成为适用于任何实验室的可扩展解决方案。
