Moos M, Goldberg N D
Department of Biochemistry, Medical School, University of Minnesota, Minneapolis 55455.
Second Messengers Phosphoproteins. 1988;12(4):163-70.
Platelets permeabilized by means of a high voltage electric field demonstrated time- and ATP-dependent uptake of 45Ca++. Submicromolar concentrations of inositol-1,4,5-trisphosphate (IP3) caused a rapid release of 45Ca++ which was followed by a slower reuptake. Adenosine 3':5'-cyclic monophosphate (cAMP) did not affect 45Ca++ uptake but did reduce IP3-mediated calcium release in a concentration-dependent manner over the range of 1-100 microM. Because cAMP concentrations in this range occur following exposure of platelets to prostacyclin and other agents which interfere with platelet function, it is proposed that cAMP-mediated inhibition of the action of IP3 may play a role in the antithrombotic activity of compounds believed to elevate levels of this cyclic nucleotide.
通过高压电场通透化处理的血小板表现出对45Ca++的时间和ATP依赖性摄取。亚微摩尔浓度的肌醇-1,4,5-三磷酸（IP3）引起45Ca++的快速释放，随后是较慢的再摄取。3':5'-环磷酸腺苷（cAMP）不影响45Ca++摄取，但在1-100 microM范围内以浓度依赖性方式减少IP3介导的钙释放。由于血小板暴露于前列环素和其他干扰血小板功能的物质后会出现此范围内的cAMP浓度，因此有人提出，cAMP介导的对IP3作用的抑制可能在被认为能提高这种环核苷酸水平的化合物的抗血栓活性中起作用。
