Yang Qian, Tang Peng, He Guangbin, Ge Shuping, Liu Liwen, Zhou Xiaodong
Department of Ultrasound, Xijing Hospital, Xi'an, China.
Department of Orthopedics and Rehabilitation, China Rehabilitation Research Center, Beijing Charity Hospital, Beijing, China.
Ultrasound Med Biol. 2017 Aug;43(8):1658-1670. doi: 10.1016/j.ultrasmedbio.2017.03.013. Epub 2017 May 22.
We investigated a new method for combining microbubble-enhanced ultrasound cavitation (MEUC) with hemocoagulase (HC) atrox. Our goal was to induce embolic effects in the vasculature and combine these with an anti-angiogenic treatment strategy. Fourteen days after being implanted with a single slice of the liver VX2 tumor, rabbits were randomly divided into five groups: (i) a control group injected intra-venously with saline using a micropump; (ii) a group given only an injection of HC; (iii) a group treated only with ultrasound cavitation; (iv) a group treated with MEUC; (v) a group treated with MEUC + HC. Contrast-enhanced ultrasound was performed before treatment and 1 h and 7 d post-treatment to measure tumor size, enhancement and necrosis range. QontraXt software was used to determine the time-intensity curve of tumor blood perfusion and microvascular changes. At 1 h and 7 d after treatment with MEUC + HC, the parameters of the time-intensity curve, which included peak value, regional blood volume, regional blood flow and area under the curve value and which were measured using contrast-enhanced ultrasound, were significantly lower than those of the other treatment groups. The MEUC + HC treatment group exhibited significant growth inhibition relative to the ultrasound cavitation only, HC and MEUC treatment groups. No damage was observed in the surrounding normal tissues. These results support the feasibility of reducing the blood perfusion of rabbit VX2 liver tumors using a new method that combines MEUC and HC.
我们研究了一种将微泡增强超声空化（MEUC）与矛头蝮蛇血凝酶（HC）相结合的新方法。我们的目标是在脉管系统中诱导栓塞效应，并将这些效应与抗血管生成治疗策略相结合。在植入一片肝脏VX2肿瘤14天后，将兔子随机分为五组：（i）对照组，使用微量泵静脉注射生理盐水；（ii）仅注射HC的组；（iii）仅接受超声空化治疗的组；（iv）接受MEUC治疗的组；（v）接受MEUC + HC治疗的组。在治疗前以及治疗后1小时和7天进行超声造影，以测量肿瘤大小、增强情况和坏死范围。使用QontraXt软件确定肿瘤血液灌注的时间-强度曲线和微血管变化。在接受MEUC + HC治疗后1小时和7天，使用超声造影测量的时间-强度曲线参数，包括峰值、局部血容量、局部血流和曲线下面积值，均显著低于其他治疗组。与仅超声空化、HC和MEUC治疗组相比，MEUC + HC治疗组表现出显著的生长抑制。在周围正常组织中未观察到损伤。这些结果支持了使用一种将MEUC和HC相结合的新方法来减少兔VX2肝肿瘤血液灌注的可行性。
