Williams Alicia K, Bacosa Hernando P, Quigg Antonietta
Texas A&M University at Galveston, Department of Marine Biology, 200 Seawolf Parkway, Galveston, TX 77554, USA; Texas A&M University, Department of Oceanography, 797 Lamar Street, College Station, TX 77840, USA.
Texas A&M University at Galveston, Department of Marine Biology, 200 Seawolf Parkway, Galveston, TX 77554, USA; The University of Texas at Austin, Marine Science Institute, 750 Channel View Drive, Port Aransas, TX 78373, USA.
Mar Pollut Bull. 2017 Aug 15;121(1-2):32-44. doi: 10.1016/j.marpolbul.2017.05.033. Epub 2017 May 22.
Ongoing bioremediation research seeks to promote naturally occurring microbial polycyclic aromatic hydrocarbon (PAH) degradation during and after oil spill events. However, complex relationships among functionally different microbial groups, nutrients and PAHs remain unconstrained. We conducted a surface water survey and corresponding nutrient amendment bioassays following the Texas City "Y" oil spill in Galveston Bay, Texas. Resident microbial groups, defined as either heterotrophic or autotrophic were enumerated by flow cytometry. Heterotrophic abundance was increased by oil regardless of nutrient concentrations. Contrastingly, autotrophic abundance was inhibited by oil, but this reaction was less severe when nutrient concentrations were higher. Several PAH compounds were reduced in nutrient amended treatments relative to controls suggesting nutrient enhanced microbial PAH processing. These findings provide a first-look at nutrient limitation during microbial oil processing in Galveston Bay, an important step in understanding if nutrient additions would be a useful bioremediation strategy in this and other estuarine systems.
正在进行的生物修复研究旨在促进石油泄漏事件期间及之后自然发生的微生物对多环芳烃（PAH）的降解。然而，功能不同的微生物群落、营养物质和多环芳烃之间的复杂关系仍未得到确定。在得克萨斯州加尔维斯顿湾发生得克萨斯城“Y”号石油泄漏事件后，我们进行了一次地表水调查以及相应的营养物质添加生物测定。通过流式细胞术对定义为异养或自养的本地微生物群落进行计数。无论营养物质浓度如何，石油都会增加异养菌的丰度。相比之下，石油会抑制自养菌的丰度，但当营养物质浓度较高时，这种反应不太严重。与对照相比，在添加营养物质的处理中，几种多环芳烃化合物减少，这表明营养物质促进了微生物对多环芳烃的处理。这些发现首次揭示了加尔维斯顿湾微生物处理石油过程中的营养限制，这是了解添加营养物质是否会成为该河口系统及其他河口系统中一种有用的生物修复策略的重要一步。
