Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil; School of Veterinary and Animal Science, Animal Pathology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.
Institute of Biological Sciences, Department of Biochemistry and Molecular Biology, Federal University of Goiás, 74001-970 Goiânia, Goiás, Brazil.
Int J Biol Macromol. 2017 Oct;103:683-691. doi: 10.1016/j.ijbiomac.2017.05.114. Epub 2017 May 22.
The dimorphic fungi Paracoccidioides spp. are the etiological agents of paracoccidioidomycosis (PCM), a prevalent systemic mycosis in Latin America. The Paracoccidioides lutzii response to oxidative stress is largely unexplored. Thioredoxins (TRX) are involved in the regulation of the redox environment in the cell, responding to oxidative stress in several organisms. In this study, we describe the isolation and characterization of a cDNA encoding a thioredoxin 1 from yeast cells from P. lutzii. The cDNA codes for a 12kDa protein containing the characteristic thioredoxin active site. The thioredoxin 1 gene was expressed in Escherichia coli and the isolated thioredoxin 1 recombinant protein as the native PlTRX1 from yeast cells showed insulin reduction activity in vitro. We also showed by semi-quantitative RT-PCR analysis that the expression of thioredoxin 1 gene was induced in response to HO and may exert an antioxidant activity in vivo. Our results suggest that the thioredoxin 1 may play an important role in controlling the redox status in P. lutzii which may contribute to this organism's virulence.
双相真菌荚膜组织胞浆菌属是荚膜组织胞浆菌病（PCM）的病原体，这是一种在拉丁美洲流行的系统性真菌病。荚膜组织胞浆菌属对氧化应激的反应在很大程度上尚未被探索。硫氧还蛋白（TRX）参与细胞内氧化还原环境的调节，在几种生物体中对氧化应激做出反应。在这项研究中，我们描述了从荚膜组织胞浆菌酵母细胞中分离和鉴定编码硫氧还蛋白 1 的 cDNA。该 cDNA 编码一个 12kDa 的蛋白质，含有特征性的硫氧还蛋白活性位点。该硫氧还蛋白 1 基因在大肠杆菌中表达，分离的硫氧还蛋白 1 重组蛋白作为来自酵母细胞的天然 PlTRX1，在体外显示胰岛素还原活性。我们还通过半定量 RT-PCR 分析表明，硫氧还蛋白 1 基因的表达被 HO 诱导，并可能在体内发挥抗氧化活性。我们的结果表明，硫氧还蛋白 1 可能在控制荚膜组织胞浆菌属的氧化还原状态中发挥重要作用，这可能有助于该生物体的毒力。
