Casaletti L, Lima P S, Oliveira L N, Borges C L, Báo S N, Bailão A M, Soares C M A
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, ICBII, Campus II, Universidade Federal de Goiás, 74001-970, Goiânia, Goiás, Brazil.
Escola de Engenharia, Pontifícia Universidade Católica de Goiás, 74605-010, Goiânia, Goiás, Brazil.
Yeast. 2017 Apr;34(4):179-188. doi: 10.1002/yea.3225. Epub 2017 Jan 26.
The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC-MS system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii, and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii, to date. Copyright © 2016 John Wiley & Sons, Ltd.
副球孢子菌属由嗜热双相真菌组成，是副球孢子菌病的病原体，副球孢子菌病是拉丁美洲最常见的系统性真菌病之一。线粒体拥有复杂的ATP生成机制，这涉及柠檬酸循环和乙醛酸循环、电子传递链和氧化磷酸化等代谢途径。此外，该细胞器在细胞中执行多种功能，作为一个特殊的代谢信号中心，参与真核生物的细胞应激反应，如自噬和凋亡。这项工作的目的是对鲁氏副球孢子菌酵母细胞中的线粒体进行描述性蛋白质组学分析。线粒体分级分离后，用胰蛋白酶消化富含线粒体蛋白质的样品，并使用纳升级超高效液相色谱-质谱系统（英国曼彻斯特的沃特世公司）进行分析。我们的结果表明，所建立的线粒体纯化方案对鲁氏副球孢子菌非常有效，在我们的分析中，有298种蛋白质被鉴定为主要存在于线粒体中。据我们所知，这是迄今为止鲁氏副球孢子菌线粒体蛋白质的首次汇编。版权所有© 2016约翰威立父子有限公司。
