Kim A Rum, Kim Kyung Min, Byun Mi Ran, Hwang Jun-Ha, Park Jung Il, Oh Ho Taek, Kim Hyo Kyeong, Jeong Mi Gyeong, Hwang Eun Sook, Hong Jeong-Ho
Department of Life Sciences, School of Life Sciences and Biotechnology, Korea University, Seoul 02841, South Korea.
College of Pharmacy, Ewha Woman's University, Seoul 03760, South Korea.
Biochem Biophys Res Commun. 2017 Jul 22;489(2):142-148. doi: 10.1016/j.bbrc.2017.05.114. Epub 2017 May 22.
Muscle weakness is one of the most common symptoms in aged individuals and increases risk of mortality. Thus, maintenance of muscle mass is important for inhibiting aging. In this study, we investigated the effect of catechins, polyphenol compounds in green tea, on muscle regeneration. We found that (-)-epicatechin gallate (ECG) and (-)-epigallocatechin-3-gallate (EGCG) activate satellite cells by induction of Myf5 transcription factors. For satellite cell activation, Akt kinase was significantly induced after ECG treatment and ECG-induced satellite cell activation was blocked in the presence of Akt inhibitor. ECG also promotes myogenic differentiation through the induction of myogenic markers, including Myogenin and Muscle creatine kinase (MCK), in satellite and C2C12 myoblast cells. Finally, EGCG administration to mice significantly increased muscle fiber size for regeneration. Taken together, the results suggest that catechins stimulate muscle stem cell activation and differentiation for muscle regeneration.
肌肉无力是老年人最常见的症状之一，会增加死亡风险。因此，维持肌肉质量对于延缓衰老很重要。在本研究中，我们调查了绿茶中的多酚类化合物儿茶素对肌肉再生的影响。我们发现，（-）-表儿茶素没食子酸酯（ECG）和（-）-表没食子儿茶素-3-没食子酸酯（EGCG）通过诱导Myf5转录因子来激活卫星细胞。对于卫星细胞的激活，ECG处理后Akt激酶被显著诱导，并且在存在Akt抑制剂的情况下，ECG诱导的卫星细胞激活被阻断。ECG还通过在卫星细胞和C2C12成肌细胞中诱导包括肌细胞生成素和肌肉肌酸激酶（MCK）在内的成肌标志物来促进成肌分化。最后，给小鼠施用EGCG显著增加了用于再生的肌纤维大小。综上所述，结果表明儿茶素刺激肌肉干细胞的激活和分化以促进肌肉再生。
