Burgasova M P, Sorokina T B, Chaplygina N M, Bogomolova N N
Vopr Virusol. 1988 Nov-Dec;33(6):703-7.
Avian adenovirus CELO was found to replicate poorly in Japanese quail embryos (JQE) and cell cultures of them. The infectious process in these systems was latent. The antigen of adenovirus CELO in JQE cell culture was detectable by the fluorescent antibody method (FAM) within the first 24-72 hours after inoculation as fluorescent cytoplasmic granules. Subsequently, fluorescence of nuclei and macrophage cytoplasm was observed. The results indicate that JQE and their cell cultures are not contaminated with avian adenovirus CELO despite regular circulation of this agent among avian populations. The advantages of FAM (rapidity and clearness) for identification of adenovirus as substrates contaminant as compared with other biological methods have been demonstrated.
发现禽腺病毒CELO在日本鹌鹑胚胎（JQE）及其细胞培养物中复制不佳。这些系统中的感染过程是潜伏性的。接种后最初24 - 72小时内，可通过荧光抗体法（FAM）在JQE细胞培养物中检测到腺病毒CELO的抗原，表现为荧光细胞质颗粒。随后，观察到细胞核和巨噬细胞细胞质出现荧光。结果表明，尽管该病原体在禽类群体中经常传播，但JQE及其细胞培养物未被禽腺病毒CELO污染。与其他生物学方法相比，已证明FAM（快速性和清晰度）在鉴定作为底物污染物的腺病毒方面的优势。
