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一种用于检测鸡体内禽腺病毒和禽腺病毒相关病毒抗体的酶联免疫吸附测定法。

An enzyme-linked immunosorbent assay for detection of antibodies to avian adenovirus and avian adenovirus-associated virus in chickens.

作者信息

Dawson G J, Orsi L N, Yates V J, Chang P W, Pronovost A D

出版信息

Avian Dis. 1980 Apr-Jun;24(2):393-402.

PMID:6254491
Abstract

An enzyme-linked immunosorbent assay system (ELISA) was adapted for the detection of antibodies to avian adenovirus (AV) and avian adenovirus-associated virus (A-AV). Both before and after exposure, sera from chickens undergoing natural and experimental infections were assayed by ELISA, virus neutralization (VN), and immunodiffusion (ID) for antibody to both CELO virus and A-AV. The ELISA system was found to be comparable to VN for determining antibody concentrations to CELO virus and A-AV. In many cases, ELISA was found to be more sensitive than ID.

摘要

一种酶联免疫吸附测定系统(ELISA)被用于检测禽腺病毒(AV)和禽腺病毒相关病毒(A - AV)的抗体。在自然感染和实验感染前后,对经历自然感染和实验感染的鸡的血清进行ELISA、病毒中和试验(VN)和免疫扩散试验(ID),以检测针对CELO病毒和A - AV的抗体。结果发现,ELISA系统在测定针对CELO病毒和A - AV的抗体浓度方面与VN相当。在许多情况下,ELISA比ID更敏感。

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