Bird R C, Nusbaum K E, Screws E A, Young-White R R, Grizzle J M, Toivio-Kinnucan M
Department of Pathobiology, College of Veterinary Medicine, Auburn University, AL 36849.
Am J Vet Res. 1988 Nov;49(11):1850-5.
Eleven EcoRI DNA fragments from the genome of an isolate of channel catfish virus (CCV) were cloned into the bacterial vector pUC19. The cloned DNA fragments ranged in size from approximately 200 base pairs to greater than 5,400 base pairs and accounted for about 13.5% of the 130,000-base pair CCV genome. Nine of these CCV DNA fragments encoded sequences that were expressed during late CCV infection. Channel catfish (total length, 4 cm) injected with CCV expressed CCV mRNA at detectable amounts in greater than or equal to 1 tissues. Uninjected control fish failed to express CCV-specific mRNA or expressed CCV-specific mRNA at lower amounts because of the presence of endogenous CCV. Tissue samples from clinically normal channel catfish fingerlings from 2 other farms as well as from adult brood stock also expressed CCV-specific mRNA. The results suggest that CCV can persist in a dormant or transcriptionally active state without causing clinical disease.
从斑点叉尾鮰病毒（CCV）分离株的基因组中获得的11个EcoRI DNA片段被克隆到细菌载体pUC19中。克隆的DNA片段大小从约200个碱基对到大于5400个碱基对不等，约占130000个碱基对的CCV基因组的13.5%。这些CCV DNA片段中的9个编码在CCV感染后期表达的序列。注射了CCV的斑点叉尾鮰（全长4厘米）在大于或等于1个组织中以可检测到的量表达CCV mRNA。未注射的对照鱼由于内源性CCV的存在，未能表达CCV特异性mRNA或表达量较低。来自另外2个养殖场的临床正常斑点叉尾鮰幼鱼以及成年亲鱼的组织样本也表达CCV特异性mRNA。结果表明，CCV可以在休眠或转录活跃状态下持续存在而不引起临床疾病。
