Wise J A, Harrell S F, Busch R L, Boyle J A
Department of Biochemistry, Mississippi State University 39762.
Am J Vet Res. 1988 Sep;49(9):1506-9.
Channel catfish broodfish were examined nondestructively for the presence of channel catfish virus (CCV), using a nucleic acid-probing technique. A dot blot assay was tested and shown to be accurate and rapid in the diagnosis of CCV. Two CCV-positive fish were successfully mated, and fertilized eggs were collected. Offspring that hatched were tested and were shown to have CCV. This result indicated vertical transmission of CCV, a herpesvirus.
采用核酸探测技术对斑点叉尾鮰亲鱼进行了非破坏性检测,以确定是否存在斑点叉尾鮰病毒(CCV)。对一种斑点杂交试验进行了测试,结果表明该试验在诊断CCV方面准确且快速。两只CCV呈阳性的鱼成功交配,并收集了受精卵。对孵化出的后代进行检测,结果显示其携带CCV。这一结果表明CCV(一种疱疹病毒)能够垂直传播。