Wan Xinyue, Yin Jieyun, Foreman Robert, Chen Jiande D Z
Veterans Research and Education Foundation, VA Medical Center, Oklahoma City, OK, USA.
Division of Gastroenterology, Wuhan University, Renmin Hospital, Wuhan, China.
Obes Surg. 2017 Dec;27(12):3215-3222. doi: 10.1007/s11695-017-2743-1.
This paper aims to optimize stimulation parameters and durations for intestinal electrical stimulation (IES) and to explore the effects and mechanisms of chronic IES with optimized methodology in obesity rats.
Sixteen diet-induced obese (DIO) rats were tested for food intake with four different sets of IES parameters each lasting 1 week. Then, another 12 DIO rats were used to test the effect of IES on food intake with different stimulation durations. Finally, 16 DIO rats were treated with IES or sham-IES for 4 weeks. Meal patterns, food intake, and body weight were observed. Mechanisms involving gastrointestinal motility, ghrelin, and glucagon-like peptide-1 (GLP-1) were studied.
(1) Acute IES with different parameters showed different inhibitory effects on food intake, and the most effective parameters were 0.6 s on, 0.9 s off, 80 Hz, 2 ms, and 4 mA with which 26.3% decrease in food intake was noted (p < 0.001). (2) IES with daily treatment of 12 h was most effective in suppressing food intake compared with 1 or 6 h. (3) Four-week IES reduced net weight by 10.9% (p < 0.05 vs. sham-IES) and epididymal fat pad weight by 13.9% (p < 0.001). (4) IES delayed gastric emptying (p < 0.001) and accelerated intestinal transit (p < 0.05). (5) IES increased both fasting and postprandial plasma levels of GLP-1 but not ghrelin.
Twelve-hour daily IES using optimized stimulation parameters reduces food intake and body weight in DIO rats by altering gastrointestinal motility and GLP-1. The IES methodology derived in this study may have a therapeutic potential for obesity.
本文旨在优化肠道电刺激（IES）的刺激参数和持续时间，并探讨采用优化方法进行慢性IES对肥胖大鼠的影响及机制。
16只饮食诱导肥胖（DIO）大鼠，采用四组不同的IES参数进行食物摄入量测试，每组持续1周。然后，另取12只DIO大鼠，用于测试不同刺激持续时间的IES对食物摄入量的影响。最后，16只DIO大鼠接受IES或假IES治疗4周。观察进食模式、食物摄入量和体重。研究涉及胃肠动力、胃饥饿素和胰高血糖素样肽-1（GLP-1）的机制。
（1）不同参数的急性IES对食物摄入量显示出不同的抑制作用，最有效的参数为开启0.6秒、关闭0.9秒、80赫兹、2毫秒和4毫安，食物摄入量减少了26.3%（p<0.001）。（2）与1小时或6小时相比，每天治疗12小时的IES在抑制食物摄入量方面最有效。（3）四周的IES使净重降低了10.9%（与假IES相比，p<0.05），附睾脂肪垫重量降低了13.9%（p<0.001）。（4）IES延迟了胃排空（p<0.001）并加速了肠道转运（p<0.05）。（5）IES增加了空腹和餐后血浆GLP-1水平，但未增加胃饥饿素水平。
使用优化刺激参数进行每日12小时的IES可通过改变胃肠动力和GLP-1来减少DIO大鼠的食物摄入量和体重。本研究得出的IES方法可能对肥胖具有治疗潜力。
