文献检索，用中文搜 PubMed

Suppr 超能文献

核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Adams P C, Mack U, Powell L W, Halliday J W

Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia.

Comp Biochem Physiol B. 1988;90(4):837-41. doi: 10.1016/0305-0491(88)90342-2.

A ferritin receptor has been isolated from porcine liver and has been partially purified using affinity chromatography. 2. A binding assay has been developed which utilizes a hepatic ferritin receptor coupled to a microparticulate support which facilitates the separation of bound and free ligand. 3. An affinity constant of 2.9 x 10(9) mol-1 litre was determined for the purified hepatic ferritin receptor. 4. The molecular weight of the receptor was estimated to be approximately 53,000 by gel electrophoresis. 5. Binding of ferritin to the insolubilized receptor was unaffected by a 100-fold excess of bovine albumin, porcine and human transferrin, and human asialo-orosomucoid. 6. Binding was specific for porcine ferritin with no demonstrable binding of rat or human ferritin.

已从猪肝中分离出铁蛋白受体，并使用亲和色谱法进行了部分纯化。2. 已开发出一种结合测定法，该方法利用与微粒支持物偶联的肝铁蛋白受体，这有助于分离结合的和游离的配体。3. 测定纯化的肝铁蛋白受体的亲和常数为2.9×10⁹ mol⁻¹升。4. 通过凝胶电泳估计该受体的分子量约为53,000。5. 铁蛋白与固定化受体的结合不受100倍过量的牛血清白蛋白、猪和人转铁蛋白以及人去唾液酸糖蛋白的影响。6. 结合对猪铁蛋白具有特异性，对大鼠或人铁蛋白无明显结合。

Adams P C, Mack U, Powell L W, Halliday J W

Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia.

Comp Biochem Physiol B. 1988;90(4):837-41. doi: 10.1016/0305-0491(88)90342-2.

A ferritin receptor has been isolated from porcine liver and has been partially purified using affinity chromatography. 2. A binding assay has been developed which utilizes a hepatic ferritin receptor coupled to a microparticulate support which facilitates the separation of bound and free ligand. 3. An affinity constant of 2.9 x 10(9) mol-1 litre was determined for the purified hepatic ferritin receptor. 4. The molecular weight of the receptor was estimated to be approximately 53,000 by gel electrophoresis. 5. Binding of ferritin to the insolubilized receptor was unaffected by a 100-fold excess of bovine albumin, porcine and human transferrin, and human asialo-orosomucoid. 6. Binding was specific for porcine ferritin with no demonstrable binding of rat or human ferritin.

已从猪肝中分离出铁蛋白受体，并使用亲和色谱法进行了部分纯化。2. 已开发出一种结合测定法，该方法利用与微粒支持物偶联的肝铁蛋白受体，这有助于分离结合的和游离的配体。3. 测定纯化的肝铁蛋白受体的亲和常数为2.9×10⁹ mol⁻¹升。4. 通过凝胶电泳估计该受体的分子量约为53,000。5. 铁蛋白与固定化受体的结合不受100倍过量的牛血清白蛋白、猪和人转铁蛋白以及人去唾液酸糖蛋白的影响。6. 结合对猪铁蛋白具有特异性，对大鼠或人铁蛋白无明显结合。