营养不良仓鼠骨骼肌中的钙蛋白酶和钙蛋白酶抑制蛋白水平。

Calpain and calpastatin levels in dystrophic hamster skeletal muscles.

Johnson P, Hammer J L

Department of Chemistry, Ohio University, Athens 45701.

Int J Biochem. 1988;20(11):1227-30. doi: 10.1016/0020-711x(88)90223-6.

Abstract

Two fast-twitch skeletal muscles from normal and dystrophic hamsters were analysed for their calpain and calpastatin contents. 2. Assays of wide-specificity calpain II showed that the activity levels in the two muscles were increased 1.5 and 1.6 times in dystrophic animals. 3. Analysis of calpastatin levels showed that the respective dystrophic muscles had activity levels of 2.2 and 2.8 times those of control muscles. 4. These results contrast with previous studies on denervated hamster muscles which showed that denervation causes an increase in calpain levels but a decrease in calpastatin levels.

摘要

对正常和患营养不良症仓鼠的两块快肌骨骼肌进行了钙蛋白酶和钙蛋白酶抑制蛋白含量分析。2. 广谱钙蛋白酶II的检测表明，在患营养不良症的动物中，这两块肌肉的活性水平分别提高了1.5倍和1.6倍。3. 钙蛋白酶抑制蛋白水平分析表明，相应的患营养不良症的肌肉活性水平分别是对照肌肉的2.2倍和2.8倍。4. 这些结果与之前对去神经支配仓鼠肌肉的研究形成对比，之前的研究表明去神经支配会导致钙蛋白酶水平升高，但钙蛋白酶抑制蛋白水平降低。