Klamut H J, Lin C H, Strickland K P
Muscle Nerve. 1983 Jul-Aug;6(6):436-41. doi: 10.1002/mus.880060607.
As part of a study on the implication of elevated Ca2+ levels in the myofibrillar degeneration seen in dystrophic muscle, the content of calmodulin and the activity of Ca2+-activated neutral protease (CANP) have been measured in normal and dystrophic (UM-X7.1) hamsters. Calmodulin levels, expressed as micrograms +/- SEM per gram wet weight were highest in brain (385 +/- 24.7), followed by tongue (93.88 +/- 3.93), heart (42.13 +/- 2.93), and skeletal muscle (31.69 +/- 1.42). No significant increases in calmodulin were observed in the dystrophic tissues thus suggesting that the Ca2+ accumulations observed in dystrophic muscles are unrelated to changes in a calmodulin levels. Because of the complexity of regulation of CANP, a time-dependent study was done using extracts of skeletal, heart, and tongue muscles. Marginal increases in dystrophic CANP were seen in skeletal muscle at all times studied and in the heart and tongue at initial time points only. The data are discussed in terms of rising levels of Ca2+ in muscles of the UM-X7.1 hamster being sufficient to increase CANP activity (without increasing content) to where it causes Z-line dissolution.
作为一项关于肌营养不良肌肉中肌原纤维变性时钙离子水平升高的影响的研究的一部分，已经测定了正常和肌营养不良（UM-X7.1）仓鼠中钙调蛋白的含量以及钙离子激活的中性蛋白酶（CANP）的活性。以每克湿重微克数±标准误表示的钙调蛋白水平在脑中最高（385±24.7），其次是舌头（93.88±3.93）、心脏（42.13±2.93）和骨骼肌（31.69±1.42）。在营养不良组织中未观察到钙调蛋白有显著增加，因此表明在营养不良肌肉中观察到的钙离子积累与钙调蛋白水平的变化无关。由于CANP调节的复杂性，使用骨骼肌、心脏和舌头肌肉提取物进行了一项时间依赖性研究。在所有研究时间点，营养不良的骨骼肌中CANP有少量增加，仅在初始时间点心脏和舌头中有增加。根据UM-X7.1仓鼠肌肉中钙离子水平升高足以将CANP活性（不增加含量）提高到导致Z线溶解的程度这一情况对数据进行了讨论。
