He Qinglin, Yin Hua, Jiang Jingjie, Bai Yanfen, Chen Ning, Liu Shaowei, Zhuang Yibin, Liu Tao
National and Local United Engineering Lab of Metabolic Control Fermentation Technology, College of Biotechnology, Tianjin University of Science and Technology , Tianjin 300457, China.
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
J Agric Food Chem. 2017 Jun 14;65(23):4691-4697. doi: 10.1021/acs.jafc.7b00981. Epub 2017 Jun 6.
Three rosmarinic acid analogs produced by recombinant Escherichia coli, two xanthones from fungi and honokiol from plants, were explored as the substrates of E. coli harboring a glucosyltransferase mutant UGT73B6 to generate phenolic glucosides. Six new and two known compounds were isolated from the fermentation broth of the recombinant strain of the feeding experiments, and the compounds were identified by spectroscopy. The biotransformation of rosmarinic acid analogs and xanthones into corresponding glucosides was presented for the first time. This study not only demonstrated the substrate flexibility of the glucosyltransferase mutant UGT73B6 toward aromatic alcohols but also provided an effective and economical method to produce phenolic glucosides by fermentation circumventing the use of expensive precursor UDP-glucose.
研究了由重组大肠杆菌产生的三种迷迭香酸类似物、两种来自真菌的呫吨酮和来自植物的厚朴酚作为携带葡糖基转移酶突变体UGT73B6的大肠杆菌的底物，以生成酚类糖苷。从饲喂实验的重组菌株发酵液中分离出六种新化合物和两种已知化合物，并通过光谱对这些化合物进行了鉴定。首次报道了迷迭香酸类似物和呫吨酮向相应糖苷的生物转化。本研究不仅证明了葡糖基转移酶突变体UGT73B6对芳香醇的底物灵活性，还提供了一种有效且经济的通过发酵生产酚类糖苷的方法，避免了使用昂贵的前体尿苷二磷酸葡萄糖。
