Wang Xia, Yang Jin, Hu Haichao, Yuan Tangyu, Zhao Yingjie, Liu Ying, Li Wei, Liu Jiaqian
College of Plant Protection, Hunan Agricultural University, Changsha 410128, China.
State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
Viruses. 2024 Mar 22;16(4):489. doi: 10.3390/v16040489.
Glycosylation, a dynamic modification prevalent in viruses and higher eukaryotes, is principally regulated by uridine diphosphate (UDP)-glycosyltransferases (UGTs) in plants. Although UGTs are involved in plant defense responses, their responses to most pathogens, especially plant viruses, remain unclear. Here, we aimed to identify UGTs in the whole genome of () and to analyze their function in Chinese wheat mosaic virus (CWMV) infection. A total of 147 were identified in . To conduct a phylogenetic analysis, the UGT protein sequences of and were aligned. The gene structure and conserved motifs of the UGTs were also analyzed. Additionally, the physicochemical properties and predictable subcellular localization were examined in detail. Analysis of cis-acting elements in the putative promoter revealed that were involved in temperature, defense, and hormone responses. The expression levels of 20 containing defense-related cis-acting elements were assessed in CWMV-infected , revealing a significant upregulation of 8 . Subcellular localization analysis of three NbUGTs (NbUGT12, NbUGT16 and NbUGT17) revealed their predominant localization in the cytoplasm of leaves, and NbUGT12 was also distributed in the chloroplasts. CWMV infection did not alter the subcellular localization of NbUGT12, NbUGT16, and NbUGT17. Transient overexpression of , , and enhanced CWMV infection, whereas the knockdown of , and inhibited CWMV infection in . These could serve as potential susceptibility genes to facilitate CWMV infection. Overall, the findings throw light on the evolution and function of .
糖基化是病毒和高等真核生物中普遍存在的一种动态修饰，在植物中主要由尿苷二磷酸（UDP）-糖基转移酶（UGT）调控。尽管UGT参与植物防御反应，但其对大多数病原体，尤其是植物病毒的反应仍不清楚。在此，我们旨在鉴定（）全基因组中的UGT，并分析它们在小麦花叶病毒（CWMV）感染中的功能。在（）中总共鉴定出147个（UGT）。为了进行系统发育分析，对（）和（）的UGT蛋白序列进行了比对。还分析了UGT的基因结构和保守基序。此外，详细研究了其理化性质和预测的亚细胞定位。对推定启动子中的顺式作用元件分析表明，（这些元件）参与温度、防御和激素反应。在感染CWMV的（）中评估了20个含有防御相关顺式作用元件的（UGT基因）的表达水平，发现其中8个显著上调。对三个NbUGT（NbUGT12、NbUGT16和NbUGT17）的亚细胞定位分析表明，它们主要定位于（）叶片的细胞质中，并且NbUGT12也分布于叶绿体中。CWMV感染并未改变NbUGT12、NbUGT16和NbUGT17的亚细胞定位。（）、（）和（）的瞬时过表达增强了CWMV感染，而（）、（）和（）的敲低则抑制了（）中的CWMV感染。这些（UGT）可作为促进CWMV感染的潜在易感基因。总体而言，这些发现揭示了（UGT）的进化和功能。
