Lundgren T, Linde A
Department of Oral Biochemistry, Faculty of Odontology, Gothenburg University, Sweden.
J Oral Pathol. 1988 Nov;17(9-10):560-3. doi: 10.1111/j.1600-0714.1988.tb01334.x.
When incubated in the presence of Na+ ions, dissected rat incisor odontoblasts extruded Ca2+ ions, as detected by tetracycline fluorimetry. Also odontoblast mitochondria, obtained by subcellular fractionation and monitored by ion-specific mini-electrode technique, were found to extrude Ca2+ upon addition of Na+ ions. These findings demonstrate the presence of Na+/Ca2+ antiports in the odontoblast plasma membrane as well as in the odontoblast mitochondrial inner membrane. In addition, evidence was found for an ATP-dependent Ca2+ ion extrusion across the plasma membrane in these cells.
当在钠离子存在的情况下孵育时，通过四环素荧光测定法检测发现，解剖后的大鼠切牙成牙本质细胞会排出钙离子。通过亚细胞分级分离获得并使用离子特异性微电极技术监测的成牙本质细胞线粒体，在添加钠离子后也被发现会排出钙离子。这些发现证明了在成牙本质细胞质膜以及成牙本质细胞线粒体内膜中存在钠/钙反向转运体。此外，还发现这些细胞中存在一种依赖于ATP的钙离子跨质膜排出过程。
