Seux D, Joffre A, Fosset M, Magloire H
Laboratoire de Dévelopmeńt et de Pathologie des Tissus Dentaires, UPR CNRS 412, Faculté d'Odontologie, Lyon, France.
Arch Oral Biol. 1994 Feb;39(2):167-70. doi: 10.1016/0003-9969(94)90113-9.
To study the presence of L-type calcium channels during the different steps of odontoblast differentiation, a specific monoclonal antibody against 1,4-dihydropyridine receptors was used in combination with avidin-biotin-peroxidase complex labelling. Staining was seen on the cell bodies of pre-odontoblasts, concentrated at the apical pole (distal portion) of functional odontoblasts and localized on cell bodies and processes of mature odontoblasts. Thus calcium channels were expressed at the onset of differentiation and maintained in the differentiated cells but with some changes of localization. It is suggested that these channels may facilitate the entry of calcium to act as a second messenger for cellular polarization or be involved in the transcellular transport of calcium to the mineralizing front.
为研究成牙本质细胞分化不同阶段L型钙通道的存在情况，使用了一种针对1,4 - 二氢吡啶受体的特异性单克隆抗体，并结合抗生物素蛋白 - 生物素 - 过氧化物酶复合物标记法。在成牙本质细胞前体细胞体上可见染色，在功能性成牙本质细胞的顶端极（远端部分）集中，且定位于成熟成牙本质细胞的细胞体和突起上。因此，钙通道在分化开始时表达，并在分化细胞中维持，但定位有一些变化。提示这些通道可能促进钙的进入，作为细胞极化的第二信使，或参与钙向矿化前沿的跨细胞转运。
