Duan Dan-Dan, Wang Ke-Xin, Zhou Yu-Zhi, Qin Xue-Mei, Gao Li, Du Guan-Hua
1 Modern Research Center for Traditional Chinese Medicine, Shanxi University , Taiyuan, PR China .
2 College of Chemistry and Chemical Engineering, Shanxi University , Taiyuan, PR China .
Rejuvenation Res. 2017 Dec;20(6):506-516. doi: 10.1089/rej.2017.1919. Epub 2017 Nov 8.
Baicalein is a flavonoid isolated from the roots of Scutellaria baicalensis Georgi. This study aimed to ascertain the effects and potential underlying mechanisms of baicalein in d-galactose (d-gal)-induced aging rat model by integration of behavior examination, biochemical detection, and H nuclear magnetic resonance (NMR)-based metabolomic approach. Our findings suggest that baicalein significantly attenuated memory decline in d-gal-induced aging model, as manifested by increasing recognition index in novel object recognition test, shortening latency time, and increasing platform crossings in Morris water maze test. Baicalein significantly inhibited the releases of inflammatory mediators such as nitric oxide, interleukin-6, interleukin-1 beta, and tumor necrosis factor-α in d-gal-induced aging model. Metabolomic study revealed that 10 endogenous metabolites in cerebral cortex were considered as potential biomarkers of baicalein for its protective effect. Further metabolic pathway analysis showed that the metabolic alterations were associated with alanine, aspartate and glutamate metabolism, glycine, serine and threonine metabolism, inositol phosphate metabolism, and energy metabolism. These data indicate that baicalein improves learning and memory dysfunction in d-gal-induced aging rats. This might be achieved through attenuation of inflammation and metabolic dysfunction.
黄芩素是从黄芩根中分离出的一种黄酮类化合物。本研究旨在通过行为学检测、生化检测以及基于氢核磁共振（NMR）的代谢组学方法，确定黄芩素对d-半乳糖（d-gal）诱导的衰老大鼠模型的影响及潜在机制。我们的研究结果表明，黄芩素显著减轻了d-gal诱导的衰老模型中的记忆衰退，表现为在新物体识别测试中识别指数增加、在莫里斯水迷宫测试中潜伏期缩短以及平台穿越次数增加。黄芩素显著抑制了d-gal诱导的衰老模型中炎症介质如一氧化氮、白细胞介素-6、白细胞介素-1β和肿瘤坏死因子-α的释放。代谢组学研究表明，大脑皮层中的10种内源性代谢物被认为是黄芩素发挥保护作用的潜在生物标志物。进一步的代谢途径分析表明，代谢改变与丙氨酸、天冬氨酸和谷氨酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、肌醇磷酸代谢以及能量代谢有关。这些数据表明，黄芩素可改善d-gal诱导的衰老大鼠的学习和记忆功能障碍。这可能是通过减轻炎症和代谢功能障碍来实现的。
