Khazai Laila, Agosto-Arroyo Emmanuel, Rosa Marilin
Department of Anatomic Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL.
Appl Immunohistochem Mol Morphol. 2018 Sep;26(8):599-604. doi: 10.1097/PAI.0000000000000507.
P40 antibody has been shown to be a more specific squamous and basal cell marker compared with p63. As detection of myoepithelial cells (MECs) plays a critical role in breast pathology, and the fact that p40 targets an isoform of p63, this study was designed to compare these antibodies in a variety of lesions, especially those with an sclerotic stroma and carcinoma in situ. All studied lesions were selected from the daily cases of the 3 authors and stained with p63, p40, and calponin immunohistochemical stains. Thirty-four cases (and 19 internal controls) were included. Seventy percent constituted sclerotic lesions (12 cases) and ductal carcinoma in situ (12 cases). P40 and p63 stained all lesions and showed a similar patchy staining pattern in 50% of ductal carcinoma in situ and sclerotic lesions. Compared with internal controls, p40 and p63 demonstrated decreased staining intensity in up to 70% and 8% of all cases, respectively, with no cross-reactivity with mesenchymal cells and minor cross-reactivity with epithelial cells. In our study, p40 did not outperform p63 as a MEC marker. p40 showed a decreased intensity in a higher number of cases (P<0.0001). In our opinion, p63 continues to be the best nuclear marker for the detection of MECs in the daily practice of breast pathology.
与p63相比，P40抗体已被证明是一种更具特异性的鳞状和基底细胞标志物。由于肌上皮细胞（MECs）的检测在乳腺病理学中起着关键作用，且p40靶向p63的一种亚型，本研究旨在比较这两种抗体在各种病变中的表现，尤其是那些具有硬化性间质和原位癌的病变。所有研究的病变均选自三位作者的日常病例，并进行p63、p40和钙调蛋白免疫组织化学染色。纳入了34例病例（以及19例内部对照）。70%为硬化性病变（12例）和导管原位癌（12例）。P40和p63对所有病变均有染色，在50%的导管原位癌和硬化性病变中显示出相似的斑片状染色模式。与内部对照相比，p40和p63在所有病例中分别有高达70%和8%的染色强度降低，与间充质细胞无交叉反应，与上皮细胞有轻微交叉反应。在我们的研究中，p40作为MEC标志物并未优于p63。p40在更多病例中显示染色强度降低（P<0.0001）。我们认为，在乳腺病理学的日常实践中，p63仍然是检测MECs的最佳核标志物。
