Ianenko A S, Bekkarevich A O, Gerasimov V A, Krylov V N
Genetika. 1988 Dec;24(12):2120-6.
Using a large group of newly isolated deletion mutants of prophage D3112 the location of all known mutations of D3112 phage was more precisely defined. The mutations affecting establishment of lysogenic state were mapped in two regions of the genome- 0-1.3 and 29-30 kb. The replicative A gene is mapped between 1.3 and 4.9 kb, the second replicative B gene being situated on the right of the A gene, between 4.9 and 9.4 kb. The C gene which is responsible for positive regulation of phage late genes' expression is mapped within the 9-12 kb region. It is suggested that promoter of the gene C is situated within the same interval. Mutations were isolated in the Lys gene which is responsible for host cell lysis. The gene is located within the interval 14-22 kb of the physical map. The order of morphogenetic genes in the late genome region was also established.
利用一大组新分离的噬菌体D3112缺失突变体，更精确地确定了D3112噬菌体所有已知突变的位置。影响溶原状态建立的突变定位在基因组的两个区域——0至1.3千碱基对和29至30千碱基对。复制性A基因定位在1.3至4.9千碱基对之间，第二个复制性B基因位于A基因右侧，在4.9至9.4千碱基对之间。负责噬菌体晚期基因表达正调控的C基因定位在9至12千碱基对区域内。推测基因C的启动子位于同一区间内。在负责宿主细胞裂解的Lys基因中分离到了突变。该基因位于物理图谱的14至22千碱基对区间内。还确定了晚期基因组区域中形态发生基因的顺序。
