Laboratory for Bacteriophages Genetics. Mechnikov Research Institute of Vaccines and Sera, 5a Maliy Kazenniy per., Moscow, Russia.
Viruses. 2012 Dec 21;5(1):15-53. doi: 10.3390/v5010015.
Pseudomonas aeruginosa is a frequent participant in wound infections. Emergence of multiple antibiotic resistant strains has created significant problems in the treatment of infected wounds. Phage therapy (PT) has been proposed as a possible alternative approach. Infected wounds are the perfect place for PT applications, since the basic condition for PT is ensured; namely, the direct contact of bacteria and their viruses. Plenty of virulent ("lytic") and temperate ("lysogenic") bacteriophages are known in P. aeruginosa. However, the number of virulent phage species acceptable for PT and their mutability are limited. Besides, there are different deviations in the behavior of virulent (and temperate) phages from their expected canonical models of development. We consider some examples of non-canonical phage-bacterium interactions and the possibility of their use in PT. In addition, some optimal approaches to the development of phage therapy will be discussed from the point of view of a biologist, considering the danger of phage-assisted horizontal gene transfer (HGT), and from the point of view of a surgeon who has accepted the Hippocrates Oath to cure patients by all possible means. It is also time now to discuss the possible approaches in international cooperation for the development of PT. We think it would be advantageous to make phage therapy a kind of personalized medicine.
铜绿假单胞菌是一种常见的伤口感染菌。多种抗生素耐药菌株的出现给感染伤口的治疗带来了重大问题。噬菌体疗法（PT）已被提议作为一种可能的替代方法。感染的伤口是 PT 应用的理想场所，因为 PT 的基本条件得到了保证；即细菌及其病毒的直接接触。在铜绿假单胞菌中有大量毒性（“裂解性”）和温和噬菌体（“溶源性”）。然而，可接受的 PT 的毒性噬菌体的数量及其可变性是有限的。此外，毒性（和温和）噬菌体的行为与它们预期的发展规范模型存在不同程度的偏差。我们将考虑一些非规范噬菌体-细菌相互作用的例子及其在 PT 中的应用可能性。此外，从生物学家的角度，考虑噬菌体辅助水平基因转移（HGT）的危险，以及从接受希波克拉底誓言的外科医生的角度，即通过一切可能的手段治愈患者，我们将讨论噬菌体治疗发展的一些最佳方法。现在也是时候讨论一下在国际合作方面发展 PT 的可能方法了。我们认为将噬菌体疗法作为一种个性化药物是有利的。
