Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China.
Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China; College of Science, Beijing University of Chemical Technology, Beijing 100029, PR China.
Bioresour Technol. 2017 Sep;239:542-545. doi: 10.1016/j.biortech.2017.05.101. Epub 2017 May 18.
The aim of this work was to study the synthesis of medium-chain length alkanes (MCLA), as bio-aviation product. To control the chain length of alkanes and increase the production of MCLA, Escherichia coli cells were engineered by incorporating (i) a chain length specific thioesterase from Umbellularia californica (UC), (ii) a plant origin acyl carrier protein (ACP) gene and (iii) the whole fatty acid synthesis system (FASs) from Jatropha curcas (JC). The genetic combination was designed to control the product spectrum towards optimum MCLA. Decanoic, lauric and myristic acid were produced at concentrations of 0.011, 0.093 and 1.657mg/g, respectively. The concentration of final products nonane, undecane and tridecane were 0.00062mg/g, 0.0052mg/g, and 0.249mg/g respectively. Thioesterase from UC controlled the fatty acid chain length in a range of 10-14 carbons and the ACP gene with whole FASs from JC significantly increased the production of MCLA.
这项工作的目的是研究中链烷烃 (MCLA) 的合成，作为生物航空产品。为了控制烷烃的链长并增加 MCLA 的产量，通过以下方式对大肠杆菌细胞进行工程改造：(i) 来自加州熊果树的具有链长特异性的硫酯酶 (UC)，(ii) 植物来源的酰基载体蛋白 (ACP) 基因，和 (iii) 来自麻疯树的整个脂肪酸合成系统 (FASs) (JC)。该基因组合旨在控制产物谱，以获得最佳的 MCLA。癸酸、月桂酸和肉豆蔻酸的浓度分别为 0.011、0.093 和 1.657mg/g。最终产物壬烷、十一烷和十三烷的浓度分别为 0.00062mg/g、0.0052mg/g 和 0.249mg/g。来自 UC 的硫酯酶控制脂肪酸链长在 10-14 个碳之间，而来自 JC 的带有完整 FASs 的 ACP 基因显著增加了 MCLA 的产量。
