来自巴伦戈茨氏类芽孢杆菌的一种新型β-1,3-1,4-葡聚糖酶的生化特性及应用
Biochemical properties and application of a novel β-1,3-1,4-glucanase from Paenibacillus barengoltzii.
作者信息
Zhang Bin, Liu Yu, Yang Hongye, Yan Qiaojuan, Yang Shaoqing, Jiang Zheng-Qiang, Li Shaozhen
机构信息
College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
出版信息
Food Chem. 2017 Nov 1;234:68-75. doi: 10.1016/j.foodchem.2017.04.162. Epub 2017 Apr 27.
A novel endo-β-1,3-1,4-glucanase gene (PbBglu16A) was cloned from Paenibacillus barengoltzii and heterogeneously expressed in Escherichia coli. The recombinant β-1,3-1,4-glucanase (PbBglu16A) was purified to homogeneity with a recovery yield of 78.6% and a specific activity of 431.8Umg. The molecular mass of PbBglu16A was estimated to be 44.0kDa by SDS-PAGE. The optimal pH and temperature of PbBglu16A were 6.0 and 55°C, respectively. The enzyme was stable within pH 3.5-9.0 and up to 55°C. PbBglu16A exhibited high substrate specificity towards barley β-glucan, oat β-glucan and lichenin. PbBglu16A showed an endo-type cleavage pattern and hydrolyzed endogenous enzyme-deactivated oat bran into β-gluco-oligosaccharides with a yield of 7.0%, which mainly consisted of trioligosaccharide and tetraoligosaccharide. Further, PbBglu16A could promote mashing with a reduced filtration time (14.0%) and viscosity (3.4%). Thus, PbBglu16A might be a promising candidate for the production of β-gluco-oligosaccharides and in brewing industry.
从巴氏芽孢杆菌中克隆出一个新的内切-β-1,3-1,4-葡聚糖酶基因（PbBglu16A），并在大肠杆菌中进行异源表达。重组β-1,3-1,4-葡聚糖酶（PbBglu16A）被纯化至同质，回收率为78.6%，比活性为431.8Umg。通过SDS-PAGE估计PbBglu16A的分子量为44.0kDa。PbBglu16A的最佳pH值和温度分别为6.0和55°C。该酶在pH 3.5-9.0范围内以及高达55°C时都很稳定。PbBglu16A对大麦β-葡聚糖、燕麦β-葡聚糖和地衣多糖表现出高底物特异性。PbBglu16A呈现内切型切割模式，将内源性酶失活的燕麦麸水解为β-葡寡糖，产率为7.0%，主要由三糖和四糖组成。此外，PbBglu16A可以促进糖化，过滤时间缩短14.0%，粘度降低3.4%。因此，PbBglu16A可能是生产β-葡寡糖和用于酿造工业的一个有前途的候选者。
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