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表达铁调节转运蛋白基因的百脉根转基因毛状根中镉积累和耐受性增强

Enhanced Cadmium Accumulation and Tolerance in Transgenic Hairy Roots of L. Expressing Iron-Regulated Transporter Gene .

作者信息

Ye Peng, Wang Menghua, Zhang Teng, Liu Xiaoyu, Jiang He, Sun Yaping, Cheng Xiyu, Yan Qiong

机构信息

College of Life Sciences and Bioengineering, School of Science, Beijing Jiaotong University, Beijing 100044, China.

Shangrao Municipal Ecological Environment Bureau, Shangrao 334000, China.

出版信息

Life (Basel). 2020 Dec 3;10(12):324. doi: 10.3390/life10120324.

Abstract

L., a hyperaccumulator of cadmium (Cd), is regarded as a promising candidate for phytoremediation of heavy metal pollution. In the present study, the hairy roots of L. were selected as a model plant system to study the potential application of Iron-regulated Transporter Gene () for the efficient phytoremediation of Cd pollution. The transgenic hairy roots of L. expressing the gene from were successfully obtained via the -mediated method. Expression of reduced Cd stress-induced phytotoxic effects. Significantly superior root growth, increased antioxidant enzyme activities, decreased reactive oxygen species (ROS) levels, and less cell apoptosis were observed in the transgenic hairy roots of L. compared to the wild-type lines under Cd stress. Enhanced Cd accumulation was also carried out in the transgenic hairy roots compared to the control (886.8 μg/g vs. 745.0 μg/g). These results provide an important understanding of the Cd tolerance mechanism of transgenic hairy roots of L., and are of particular importance to the development of a transgenic candidate for efficient phytoremediation process.

摘要

L.是一种镉(Cd)超积累植物,被视为重金属污染植物修复的一个有前景的候选者。在本研究中,选择L.的毛状根作为模型植物系统,以研究铁调节转运蛋白基因()在镉污染高效植物修复中的潜在应用。通过介导法成功获得了表达来自的基因的L.转基因毛状根。的表达降低了镉胁迫诱导的植物毒性效应。与镉胁迫下的野生型品系相比,在L.转基因毛状根中观察到显著更好的根系生长、增加的抗氧化酶活性、降低的活性氧(ROS)水平和更少的细胞凋亡。与对照相比,转基因毛状根中的镉积累也有所增强(886.8μg/g对745.0μg/g)。这些结果为L.转基因毛状根的镉耐受机制提供了重要认识,对开发用于高效植物修复过程的转基因候选物尤为重要。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcd1/7761695/7943b8214f5a/life-10-00324-g001.jpg

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