Department of Oral Surgery, University of Murcia, Murcia, Spain.
In Private Dental Practice, Murcia, Spain.
J Craniomaxillofac Surg. 2017 Aug;45(8):1251-1257. doi: 10.1016/j.jcms.2017.04.006. Epub 2017 Apr 18.
To evaluate the cytoprotective effects of melatonin (MLT) on zoledronic acid (ZA)-treated human osteoblasts.
Human osteoblasts were exposed to ZA (1, 5, 10, 50, 100 and 300 μM) and MLT (1, 10, 50, 100 y 200 μM) for 24, 48 and 72 h of incubation, to evaluate their effects on cell viability.
As ZA concentration increased, greater reductions in cell viability of human osteoblasts were induced whether at 24, 48 or 72 h incubation. At 24 h incubation with MLT, greatest cell viability was obtained when low dose of MLT was applied (without significant differences); 48 and 72 h incubation presented the greatest cell viability with the highest MLT concentrations (100 and 200 μM). MLT at concentrations of 100 and 200 μM would appear to have a certain cytoprotective effect on ZA-treated human osteoblasts with low concentrations of ZA (1 y 5 μM), whether at 24, 48 or 72 h; however, at ZA concentrations ≥10 μM the possible cytoprotective effects of MLT were low at 24 h incubation.
MLT has a cytoprotective effect on ZA-treated human osteoblasts and could represent a promising preventative alternative for patients at risk of bisphosphonate-related osteonecrosis of the jaw.
评估褪黑素（MLT）对唑来膦酸（ZA）处理的人成骨细胞的细胞保护作用。
将人成骨细胞暴露于 ZA（1、5、10、50、100 和 300μM）和 MLT（1、10、50、100 和 200μM）中 24、48 和 72h，以评估它们对细胞活力的影响。
随着 ZA 浓度的增加，人成骨细胞的活力无论在 24、48 还是 72h 孵育时均显著降低。在 24h 孵育时，MLT 的低剂量应用（无显著差异）获得最大的细胞活力；48 和 72h 孵育时，MLT 的最高浓度（100 和 200μM）获得最大的细胞活力。浓度为 100 和 200μM 的 MLT 似乎对低浓度 ZA（1 和 5μM）处理的人成骨细胞具有一定的细胞保护作用，无论在 24、48 还是 72h；然而，在 ZA 浓度≥10μM 时，MLT 的可能细胞保护作用在 24h 孵育时较低。
MLT 对 ZA 处理的人成骨细胞具有细胞保护作用，可能成为有发生双膦酸盐相关性颌骨坏死风险患者的一种有前途的预防选择。
