Basso Fernanda Gonçalves, Turrioni Ana Paula Silveira, Soares Diana Gabiela, Bagnato Vanderlei Salvador, Hebling Josimeri, de Souza Costa Carlos Alberto
Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Araraquara, SP, 14801-903, Brazil.
Support Care Cancer. 2014 Oct;22(10):2741-8. doi: 10.1007/s00520-014-2267-3. Epub 2014 May 7.
Clinical studies have shown that low-level laser therapy (LLLT) can improve local tissue healing of bisphosphonate-induced osteonecrosis of the jaw. However, the effects of laser irradiation on bisphosphonate-treated osteoblasts have not been completely elucidated.
Human osteoblasts were cultured in plain culture medium (DMEM). After 48 h, plain DMEM was replaced by DMEM with no fetal bovine serum, for a 24-h incubation followed by addition of zoledronic acid (5 μM) for additional 48 h. Cells were subjected to LLLT (InGaAsP; 780 ± 3 nm; 0.025 W) at 0.5, 1.5, 3, 5, and 7 J/cm(2), three times every 24 h. Cell viability, total protein production, alkaline phosphatase activity (ALP), mineral nodule formation, gene expression of collagen type I and ALP, and cell morphology were evaluated.
LLLT at 0.5 J/cm(2) increased cell viability of cultured osteoblasts. ALP activity and gene expression, in addition to mineral nodule formation and Col-I gene expression, were not increased by LLLT. LLLT applied to ZA-treated cells increased Col-I expression at 0.5, 1.5, and 3 J/cm(2) but did not improve any other cell activity assessed.
LLLT showed limited effects on bisphosphonate-treated osteoblasts.
临床研究表明，低强度激光治疗（LLLT）可改善双膦酸盐诱导的颌骨骨坏死的局部组织愈合。然而，激光照射对双膦酸盐处理的成骨细胞的影响尚未完全阐明。
将人成骨细胞培养于普通培养基（DMEM）中。48小时后，用不含胎牛血清的DMEM替换普通DMEM，孵育24小时，然后加入唑来膦酸（5μM）再孵育48小时。细胞分别接受能量密度为0.5、1.5、3、5和7 J/cm²的LLLT（InGaAsP；780±3 nm；0.025 W），每24小时照射3次。评估细胞活力、总蛋白产量、碱性磷酸酶活性（ALP）、矿化结节形成、I型胶原蛋白和ALP的基因表达以及细胞形态。
0.5 J/cm²的LLLT可提高培养的成骨细胞的活力。LLLT并未增加ALP活性和基因表达，以及矿化结节形成和Col-I基因表达。应用于经唑来膦酸处理的细胞的LLLT在能量密度为0.5、1.5和3 J/cm²时增加了Col-I表达，但未改善所评估的任何其他细胞活性。
LLLT对双膦酸盐处理的成骨细胞显示出有限的作用。
