Busquets Antonio, Gomila Margarita, Beiki Farid, Mulet Magdalena, Rahimian Heshmat, García-Valdés Elena, Lalucat Jorge
Department of Biology-Microbiology, Universitat de les Illes Balears, Crtra. Valldemossa km 7.5, 07122 Palma de Mallorca, Spain.
Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.
Syst Appl Microbiol. 2017 Jul;40(5):266-273. doi: 10.1016/j.syapm.2017.04.002. Epub 2017 May 11.
In a screening by multilocus sequence analysis of Pseudomonas strains isolated from diverse origins, 4 phylogenetically closely related strains (FBF58, FBF102, FBF103, and FBF122) formed a well-defined cluster in the Pseudomonas syringae phylogenetic group. The strains were isolated from citrus orchards in northern Iran with disease symptoms in the leaves and stems and its pathogenicity against citrus plants was demonstrated. The whole genome of the type strain of the proposed new species (FBF102=CECT 9164=CCUG 69273) was sequenced and characterized. Comparative genomics with the 14 known Pseudomonas species type strains of the P. syringae phylogenetic group demonstrated that this strain belonged to a new genomic species, different from the species described thus far. Genome analysis detected genes predicted to be involved in pathogenesis, such as an atypical type 3 secretion system and two type 6 secretion systems, together with effectors and virulence factors. A polyphasic taxonomic characterization demonstrated that the 4 plant pathogenic strains represented a new species, for which the name Pseudomonas caspiana sp. nov. is proposed.
在一项对从不同来源分离的假单胞菌菌株进行多位点序列分析的筛选中，4株亲缘关系密切的菌株（FBF58、FBF102、FBF103和FBF122）在丁香假单胞菌系统发育组中形成了一个明确的聚类。这些菌株是从伊朗北部的柑橘园中分离出来的，叶片和茎部有病害症状，并且证明了它们对柑橘植物具有致病性。对拟议新物种的模式菌株（FBF102 = CECT 9164 = CCUG 69273）的全基因组进行了测序和表征。与丁香假单胞菌系统发育组的14种已知假单胞菌物种模式菌株进行比较基因组学分析表明，该菌株属于一个新的基因组物种，与迄今描述的物种不同。基因组分析检测到预测参与致病过程的基因，如一个非典型的III型分泌系统和两个VI型分泌系统，以及效应子和毒力因子。多相分类学特征表明，这4株植物致病菌株代表一个新物种，为此提出了新种名为“Pseudomonas caspiana sp. nov.”。
